PMID: 9008705Jan 13, 1997Paper

An early stage of membrane fusion mediated by the low pH conformation of influenza hemagglutinin depends upon membrane lipids

The Journal of Cell Biology
L V ChernomordikJ Zimmerberg

Abstract

While the specificity and timing of membrane fusion in diverse physiological reactions, including virus-cell fusion, is determined by proteins, fusion always involves the merger of membrane lipid bilayers. We have isolated a lipid-dependent stage of cell-cell fusion mediated by influenza hemagglutinin and triggered by cell exposure to mildly acidic pH. This stage preceded actual membrane merger and fusion pore formation but was subsequent to a low pH-induced change in hemagglutinin conformation that is required for fusion. A low pH conformation of hemagglutinin was required to achieve this lipid-dependent stage and also, downstream of it, to drive fusion to completion. The lower the pH of the medium applied to trigger fusion and, thus, the more hemagglutinin molecules activated, the less profound was the dependence of fusion on lipids. Membrane-incorporated lipids affected fusion in a manner that correlated with their dynamic molecular shape, a characteristic that determines a lipid monolayer's propensity to bend in different directions. The lipid sensitivity of this stage, i.e., inhibition of fusion by inverted cone-shaped lysophosphatidylcholine and promotion by cone-shaped oleic acid, was consistent with the stalk hypothesis...Continue Reading

References

Aug 20, 1979·Biochimica Et Biophysica Acta·H U Weltzien
Nov 6, 1992·Science·J M White
Dec 1, 1992·The Journal of Cell Biology·J R Monck, J M Fernandez
Jan 1, 1991·Annals of the New York Academy of Sciences·J ZimmerbergF S Cohen
Nov 27, 1989·Biochimica Et Biophysica Acta·K BröringB Deuticke
Jan 1, 1989·Methods in Cell Biology·H EllensJ M White
Jul 1, 1985·The Journal of Cell Biology·S J DoxseyJ White
Jan 1, 1987·Annual Review of Biochemistry·D C Wiley, J J Skehel
Oct 5, 1987·Biochimica Et Biophysica Acta·L V ChernomordikY A Chizmadzhev
Jun 18, 1985·Biochemistry·T StegmannJ Wilschut
May 1, 1983·Quarterly Reviews of Biophysics·J WhiteA Helenius
Jul 27, 1983·Biochimica Et Biophysica Acta·K HongD Papahadjopoulos
Jan 4, 1982·Biochimica Et Biophysica Acta·T D Madden, P R Cullis
Jul 1, 1982·Journal of Virology·A YoshimuraS Ohnishi
Dec 8, 1995·The Journal of Biological Chemistry·S Günther-AusbornT Stegmann
Aug 1, 1995·Current Opinion in Cell Biology·M Lindau, W Almers
Jul 1, 1995·The Journal of Membrane Biology·L ChernomordikJ Zimmerberg
Jul 26, 1995·Biochimica Et Biophysica Acta·J M Leenhouts, B De Kruijff
May 1, 1995·Journal of Virology·L ChernomordikJ Zimmerberg
Feb 22, 1994·Biochemistry·P L YeagleT D Flanagan
Jan 1, 1993·Annual Review of Biophysics and Biomolecular Structure·J ZimmerbergL V Chernomordik

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Citations

Jun 21, 2005·Archives of Biochemistry and Biophysics·Austin BaileyLeonid V Chernomordik
Jun 14, 2002·Archives of Biochemistry and Biophysics·P I Castagnet, N M Giusto
Mar 1, 2003·Biophysical Journal·Lin Yang, Huey W Huang
Oct 24, 2002·Journal of Virological Methods·B C E P D OliveiraM C Cabral
Jun 6, 2000·Advanced Drug Delivery Reviews·G Cevc, H Richardsen
Jun 6, 2000·Advanced Drug Delivery Reviews·I MartinR M Epand
May 18, 2001·Trends in Cell Biology·J Zimmerberg
Jun 6, 2000·Trends in Biochemical Sciences·C Montecucco, O Rossetto
Dec 17, 2008·Quarterly Reviews of Biophysics·Heiko Heerklotz
Apr 4, 2002·Traffic·Michael M Kozlov, Leonid V Chernomordik
Sep 13, 2005·Nature Immunology·Eugenia LeikinaLeonid V Chernomordik
Mar 27, 2004·Nature Reviews. Microbiology·Dimiter S Dimitrov
Jul 4, 2008·Nature Structural & Molecular Biology·Leonid V Chernomordik, Michael M Kozlov
Feb 23, 2010·Nature Structural & Molecular Biology·Marija VrljicAxel T Brunger
Oct 3, 2008·Proceedings of the National Academy of Sciences of the United States of America·Daniel L FloydAntoine M van Oijen
Oct 6, 1998·The Journal of General Physiology·V I RazinkovF S Cohen
Apr 30, 2008·The Journal of General Physiology·Subrata BiswasJoshua Zimmerberg
Jul 11, 2000·Molecular Biology of the Cell·E Leikina, L V Chernomordik
Oct 12, 2010·Molecular Biology of the Cell·Boris FichtmanDouglass J Forbes
Apr 16, 1999·The EMBO Journal·M Otter-NilssonT Nilsson
Nov 2, 2002·The EMBO Journal·Eugenia LeikinaLeonid V Chernomordik
Aug 13, 2009·Clinical and Experimental Pharmacology & Physiology·Kendra L FurberJens R Coorssen
Nov 12, 2003·Journal of Virology·Xiangjie Sun, Gary R Whittaker
Feb 28, 2006·Journal of Virology·Séverine BärMarc Alizon
Jun 8, 2001·Annual Review of Biochemistry·D M Eckert, P S Kim
Oct 7, 2003·Annual Review of Biochemistry·Leonid V Chernomordik, Michael M Kozlov
Apr 13, 2002·Analytical Biochemistry·Aditya MittalLeonid V Chernomordik
Jun 21, 2001·Proceedings of the National Academy of Sciences of the United States of America·P T IvanovaH A Brown
Aug 1, 2007·Proceedings of the National Academy of Sciences of the United States of America·Youngsoo Jun, William Wickner
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·D Z Cleverley, J Lenard
Feb 7, 1998·Proceedings of the National Academy of Sciences of the United States of America·A ChanturiyaJ Zimmerberg
Jul 9, 2014·Annual Review of Cell and Developmental Biology·Benjamin Podbilewicz
Nov 3, 2014·Annual Review of Virology·Margaret Kielian

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