PMID: 6536827Oct 1, 1984Paper

An easily constructed and inexpensive three-layer cassette for tritium-sensitive film autoradiography

Journal of Pharmacological Methods
C A Altar

Abstract

This report describes the design, construction, and use of a three-layer photographic cassette that is suitable for tritium-sensitive film autoradiography. The cassette can be produced easily and at a fraction of the cost of commercially available cassettes. The three-tiered design allows for the simultaneous exposure of 108 standard-sized glass microscope slides to three 20 X 34 cm pieces of 3H-Ultrofilm (LKB). The small between- or within-film variation of gray values recorded for either background, tritium-containing standards, or neighboring brain sections labeled with 3H-spiroperidol demonstrates the reliability of the cassette for autoradiographic quantification of tritium label.

References

Jul 1, 1982·Journal of Neuroscience Methods·J R UnnerstallJ M Palacios
Jan 1, 1982·Journal of Neuroscience Methods·T C RainbowB S McEwen
Aug 1, 1982·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·M Herkenham, C B Pert
Jan 1, 1982·Neuroscience and Biobehavioral Reviews·C R GallistelM Negin
Mar 1, 1984·Journal of Neuroscience Methods·C A AltarJ F Marshall

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