An easy and rapid method using microscopy to determine herbicide effects in Poaceae weed species

Pest Management Science
M HjorthH W Ravn

Abstract

A new, easy, rapid and relatively inexpensive method using microscopy has been developed for the detection of herbicide effects in leaves of grass weed species displaying no visual signs of damage. The method has potential to be used as a tool to indicate future death of grass species due to herbicide exposure by observing phytochemical effects, i.e. early-warning effects. In the present study, Apera spica-venti (L.) Beauv., Bromus hordeaceus L., Alopecurus myosuroides Huds., Lolium perenne L. and Poa annua L. were exposed to lethal rates of four herbicides with different mode of action. The herbicides investigated were the thiocarbamate: prosulfocarb, the sulfonylurea: iodosulfuron, the aryloxyphenoxypropionate: fenoxaprop-P-ethyl and the organophosphate glyphosate. Autofluorescence of leaves was studied under a microscope using ultraviolet and blue light. The fluorescence of leaves treated to enhance flavonoids was also examined. To confirm the results, microspectrofluorometry was performed. Effects indicating future death of the grasses were observed in visually healthy leaves following treatment with prosulfocarb, glyphosate and iodosulfuron. No changes were detected following treatment with fenoxaprop-P-ethyl. After exposu...Continue Reading

References

Nov 13, 1987·Biochemical and Biophysical Research Communications·J D BurtonD L Wyse
Jan 1, 1971·Archives of Biochemistry and Biophysics·J L Harwood, P K Stumpf

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Citations

Nov 25, 2019·Environmental Science and Pollution Research International·Damien A DevaultMathilde Merlo

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