An easy, rapid, and sensitive method for detection of drug-resistant influenza virus by using a sialidase fluorescent imaging probe, BTP3-Neu5Ac

PloS One
Daisuke KatoTakashi Suzuki

Abstract

Immunochromatographic kits and RT-PCR are widely used as diagnostic tools for influenza detection in clinical and hygiene fields. Immunochromatographic kits are useful for differential typing of influenza A and influenza B but cannot show if the detected virus strains have acquired drug resistance against neuraminidase inhibitors that target sialidase activity of viral neuraminidase. Although RT-PCR enables determination of drug-resistant mutants, its efficacy is limited to viruses carrying a known substitution in their neuraminidase genome sequence. In the present study, an easy, rapid and sensitive method for detection of drug-resistant influenza viruses regardless of major antigenic changes or genomic mutations was developed. By using the method in combination with virus-concentrated membranes in centrifugal filter units and a sialidase imaging probe, 2-(benzothiazol-2-yl)-4-bromophenyl-N-acetylneuraminic acid (BTP3-Neu5Ac), sialidase activity of influenza neuraminidase was visualized on membranes by the green fluorescence of produced hydrophobic BTP3 under UV irradiation with a handheld UV flashlight. Fluorescence images in the presence or absence of neuraminidase inhibitors clearly discriminated drug-resistant influenza vi...Continue Reading

References

Dec 1, 1971·The Journal of General Virology·N J Dimmock
Feb 1, 1994·The Journal of General Virology·W P BurmeisterR W Ruigrok
Feb 7, 1998·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·A L DrewsS B Greenberg
Mar 11, 2000·Lancet·L V GubarevaF G Hayden
Jan 10, 2002·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·F G Hayden
Mar 19, 2002·Antimicrobial Agents and Chemotherapy·Y FurutaK Shiraki
Apr 11, 2003·Vaccine·Catherine Gerdil
Apr 18, 2003·Luminescence : the Journal of Biological and Chemical Luminescence·Komandoor E AchyuthanCraig D Shimasaki
May 15, 1964·Science·W L DAVIESC E HOFFMANN
May 28, 2004·Virus Research·Takehiro TogashiTsuneo Morishima
Sep 6, 2005·Journal of Virology·Takashi SuzukiYasuo Suzuki
Jan 13, 2006·The Journal of Biological Chemistry·Lawrence H Pinto, Robert A Lamb
Apr 5, 2007·JAMA : the Journal of the American Medical Association·Shuji HatakeyamaYoshihiro Kawaoka
Apr 17, 2008·Respirology : Official Journal of the Asian Pacific Society of Respirology·Timothy M Uyeki
Jul 1, 2009·Infection and Immunity·Dane ParkerAlice Prince
Dec 8, 2009·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Tatiana BaranovichUNKNOWN Japanese Influenza Collaborative Study Group
Apr 20, 2010·The International Journal of Biochemistry & Cell Biology·Megan S HarrisonAnthony P Schmitt
Sep 23, 2010·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Ha T NguyenLarisa V Gubareva
Oct 1, 2010·The New England Journal of Medicine·Erhard van der VriesCharles A B Boucher
Aug 2, 2011·Emerging Infectious Diseases·AliReza EshaghiJonathan B Gubbay
Mar 6, 2012·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Mark L MeterskyTimothy Babinchak
Mar 13, 2012·Influenza and Other Respiratory Viruses·Satoko SunagawaJiro Fujita
Jan 4, 2013·Influenza and Other Respiratory Viruses·Jennifer L McKimm-Breschkin
Mar 26, 2013·Antiviral Research·Mélanie SamsonGuy Boivin
Apr 23, 2013·Glycoconjugate Journal·Tadanobu TakahashiTakashi Suzuki
Jan 18, 2014·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·E TakashitaM Tashiro
May 3, 2014·Scientific Reports·Yuuki KurebayashiTakashi Suzuki
Jul 22, 2014·Journal of Medical Virology·Vanessa CorreiaHelena Rebelo-de-Andrade
Aug 1, 2014·Microbiology and Immunology·Yuko Sakai-TagawaYoshihiro Kawaoka
Sep 23, 2014·Journal of Virological Methods·Tadanobu TakahashiTakashi Suzuki
Sep 30, 2014·Trends in Microbiology·Tokiko WatanabeYoshihiro Kawaoka
Aug 4, 2015·Biological & Pharmaceutical Bulletin·Tadanobu TakahashiTakashi Suzuki

❮ Previous
Next ❯

Citations

Apr 4, 2021·International Journal of Molecular Sciences·Akira MinamiTakashi Suzuki
May 24, 2019·ACS Chemical Biology·Yuuki KurebayashiTakashi Suzuki

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
Fluorescence
fluorescence imaging

Software Mentioned

GraphPad Prism

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.