An ECM-bound, PDGF-like growth factor and a TGF-alpha-like growth factor are required for gastrulation and spiculogenesis in the Lytechinus embryo

Developmental Biology
V GovindarajanC R Tomlinson

Abstract

Growth factors and the extracellular matrix have been shown to fulfill vital developmental roles in many embryonic systems. Our hypothesis is that a developmental role played by the extracellular matrix in sea urchins may be the binding of a PDGF-like growth factor to promote signaling activity. We report here that anti-human PDGF-B antibodies and anti-human TGF-alpha antibodies immunoprecipitated specific proteins isolated from Lytechinus embryos. Addition of these antibodies to Lytechinus embryos inhibited gastrulation and spiculogenesis. The embryos are sensitive to the antibodies from the four-cell through the hatching blastula stages, which suggests that the TGF-alpha-like and PDGF-like ligands are required for the early differentiation of the gut and spicules. We present evidence that the PDGF-like growth factor depends on the extracellular matrix for signaling activity. Synthetic peptides representing the heparan sulfate proteoglycan binding sequence on human PDGF-B were added to Lytechinus embryo cultures to compete for binding sites with the endogenous PDGF-like growth factor. The experimental peptide inhibited gastrulation and caused radially arranged multiple spicules to form. Development was unaffected by a control ...Continue Reading

Citations

Jul 14, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·G M Wessel, A Wikramanayake
Jan 26, 2000·Development, Growth & Differentiation·M CervelloR Gambino
Aug 15, 2002·Development, Growth & Differentiation·Janice Mayne, John J Robinson
Sep 3, 2003·Development, Growth & Differentiation·Mayuko Hamada, Masato Kiyomoto
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·K Symes, M Mercola
Sep 16, 2004·Development, Growth & Differentiation·Tetsuya Kominami, Hiromi Takata
Apr 1, 1998·Zoological Science·M SakumaK Yamasu
Oct 10, 2006·Development, Growth & Differentiation·Laura A Romano, Gregory A Wray
Mar 26, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C A BradhamD R McClay
May 1, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·J PayneM Mercola
Jan 18, 2007·Evolution & Development·Alan C LoveRudolf A Raff
Sep 30, 2000·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·J FloodJ J Robinson
Aug 31, 2001·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·C Sharpe, J J Robinson
Apr 6, 2004·Developmental Biology·Montserrat Fernandez-SerraMaria I Arnone

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.