An ECV304 monoculture model for permeability assessment of blood-brain barrier

Neurological Research
Shu YangZhigang Zhao

Abstract

ECV304/C6 co-culture model is a widely used tool for BBB studies. However, cell source may influence the establishment of co-culture model and some C6 cells could damage the barrier integrity. Here, we established an ECV304 monoculture model and evaluated it in the respect of tightness, tight junction proteins and discriminative brain penetration. The tightness of ECV304 cell layers was evaluated by the measurement of permeability to hydrophilic marker Lucifer yellow. Immunofluorescence method was explored to detect the expression of tight junction proteins occludin, claudin-5 and ZO-1 in ECV304 cells. The discriminative brain penetration of the model was assessed by a permeability testing of compounds with different penetration rates, including digoxin, quinidine, and propranolol. The ECV304 monolayers developed low permeability to Lucifer yellow (permeability coefficient: 0.31 ± 0.02 × 10-3 cm/min) and exhibited positive immunostaining of occludin, claudin-5 and ZO-1. The permeability coefficients of high permeable quinidine and propranolol across ECV304 cell layers were higher than that of low permeable digoxin by 3.6 and 2.8-fold, respectively. The ECV304 monoculture model developed tight paracellular barrier and discrimina...Continue Reading

References

Mar 19, 2003·Journal of Neurochemistry·Kuresh A YoudimCatherine Rice-Evans
Jan 8, 2004·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Xingrong LiuBill J Smith
Sep 7, 2005·The Journal of Surgical Research·Yongyi FanMichael Mulholland
Jul 13, 2007·International Journal of Pharmaceutics·Simone CarraraChiara Bigogno
Dec 30, 2008·Neurochemistry International·Shinsuke NakagawaMasami Niwa
Aug 6, 2010·Brain Research Reviews·Filipa Lourenço CardosoMaria Alexandra Brito
Jan 9, 2014·Neuroscience Letters·Benjamin W KrauseLaura M Rowland
Mar 20, 2014·Molecular Pharmaceutics·Imola Wilhelm, István A Krizbai
Feb 13, 2016·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Hans C HelmsBirger Brodin

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Citations

Jun 4, 2021·Cancer Management and Research·Yihao WangWei Xiang
May 21, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·M Alonso-GarridoL Manyes

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