An ECVAG trial on assessment of oxidative damage to DNA measured by the comet assay.

Mutagenesis
Clara JohanssonLennart Möller

Abstract

The increasing use of single cell gel electrophoresis (the comet assay) highlights its popularity as a method for detecting DNA damage, including the use of enzymes for assessment of oxidatively damaged DNA. However, comparison of DNA damage levels between laboratories can be difficult due to differences in assay protocols (e.g. lysis conditions, enzyme treatment, the duration of the alkaline treatment and electrophoresis) and in the end points used for reporting results (e.g. %DNA in tail, arbitrary units, tail moment and tail length). One way to facilitate comparisons is to convert primary comet assay end points to number of lesions/10(6) bp by calibration with ionizing radiation. The aim of this study was to investigate the inter-laboratory variation in assessment of oxidatively damaged DNA by the comet assay in terms of oxidized purines converted to strand breaks with formamidopyrimidine DNA glycosylase (FPG). Coded samples with DNA oxidation damage induced by treatment with different concentrations of photosensitizer (Ro 19-8022) plus light and calibration samples irradiated with ionizing radiation were distributed to the 10 participating laboratories to measure DNA damage using their own comet assay protocols. Nine of 10 ...Continue Reading

References

Oct 19, 1976·Biochemistry·K W KohnC A Friedman
Mar 29, 2000·Environmental and Molecular Mutagenesis·R R TiceY F Sasaki
Dec 11, 2002·Mutagenesis·A HartmannUNKNOWN 4th International Comet Assay Workshop
Apr 10, 2003·Free Radical Biology & Medicine·UNKNOWN European Standards Committee on Oxidative DNA Damage (ESCODD)
Apr 21, 2006·Basic & Clinical Pharmacology & Toxicology·Peter Møller
Nov 23, 2006·Mutation Research·Brian BurlinsonUNKNOWN In Vivo Comet Assay Workgroup, part of the Fourth International Workgroup on Genotoxicity Testing
Mar 28, 2009·International journal for vitamin and nutrition research. Internationale Zeitschrift für Vitamin- und Ernährungsforschung. Journal international de vitaminologie et de nutrition·Clara JohanssonLennart Möller

❮ Previous
Next ❯

Citations

Jun 22, 2010·Analytical and Bioanalytical Chemistry·Elijah J Petersen, Bryant C Nelson
Jul 20, 2010·Analytical and Bioanalytical Chemistry·Hanna L Karlsson
Aug 18, 2010·Mutagenesis·Patrizia RisoMarisa Porrini
Jul 31, 2012·Mutagenesis·Lykke ForchhammerPeter Møller
Dec 6, 2014·Mutation Research. Reviews in Mutation Research·Peter MøllerSteffen Loft
May 21, 2013·Archives of Toxicology·Amaya Azqueta, Andrew R Collins
Nov 29, 2011·Free Radical Research·Peter MøllerSteffen Loft
Jan 11, 2011·Trends in Pharmacological Sciences·Barry Halliwell
May 19, 2010·Mutation Research·Ilse DecordierMicheline Kirsch-Volders
Dec 19, 2013·Environmental and Molecular Mutagenesis·M GuérardU Plappert-Helbig
Jun 4, 2014·Environmental and Molecular Mutagenesis·Annie JensenPeter Møller
Mar 29, 2012·Particle and Fibre Toxicology·Lykke ForchhammerSteffen Loft
Nov 5, 2013·Mutation Research. Reviews in Mutation Research·Andrew CollinsUNKNOWN ComNet project
Apr 27, 2013·Biochimica Et Biophysica Acta·Andrew R Collins
Sep 10, 2014·Environmental and Molecular Mutagenesis·Peter MøllerSteffen Loft
Dec 25, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Amaya AzquetaAndrew R Collins
Jan 26, 2013·Critical Reviews in Toxicology·Peter MøllerSteffen Loft
May 9, 2015·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Günter SpeitDan D Levy
Oct 25, 2011·Free Radical Biology & Medicine·Annie JensenPeter Møller
Jun 11, 2011·Free Radical Biology & Medicine·Kamla Al-SalmaniMarcus S Cooke
Dec 22, 2014·American Journal of Preventive Medicine·Vengatesh GanapathyLurdes Queimado
Mar 22, 2015·Mutation Research. Reviews in Mutation Research·M GuérardR Froetschl
Aug 8, 2014·Frontiers in Genetics·Peter MøllerAndrew Collins
Nov 5, 2014·Frontiers in Genetics·Andrew R CollinsAmaya Azqueta
Aug 10, 2018·Environmental and Molecular Mutagenesis·Hervé PerdryElisa Boutet-Robinet

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis

Software Mentioned

SPSS
COMICS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.