An EDTA-associated anti-B agglutinin: the role of ionized calcium

Transfusion
H YasudaM Uchikawa

Abstract

It is believed that EDTA-dependent panagglutination is associated with free carboxylic acids that support reactions of rare autoagglutinins. An ABO typing discrepancy occurred in an 88-year-old patient. The specificity of his autoagglutinin was demonstrated by panel cell study and absorption tests using normal donors' red cells or immunoadsorbents coated with A, B, or O substances. Inhibition assays were performed to determine whether the autoagglutinin was inhibited by ionized calcium or carboxylic acids. The autoagglutinin had anti-B specificity when tested in the presence of EDTA. It was neutralized by group B secretor saliva and adsorbed by crystalline silica coated with simple B substances with or without EDTA, although it was absorbed by group B red cells only in the presence of EDTA. The agglutinating activity was stronger at 25 degrees C (titer 64) than at 37 degrees C (titer 16) and was destroyed by treatment of the serum with dithiothreitol, which suggests that the autoagglutinin is IgM. This activity also appeared in the patient's serum after dialysis and in an eluate obtained after adsorption with simple B substances, and it was inhibited by the addition of CaCl2 at 0.5 mM or higher concentrations. This suggests tha...Continue Reading

Citations

May 26, 1999·Vox Sanguinis·H VrielinkP N Lelie
Jun 6, 2009·Veterinary Clinical Pathology·Deanna M W SchaeferNicole Belcher
Jun 1, 2013·Journal of Clinical Pathology·Jose Manuel VagaceGuillermo Gervasini
May 9, 2007·Clinical Chemistry and Laboratory Medicine : CCLM·Giuseppe BanfiGiuseppe Lippi

Related Concepts

Blood Group H Type 1 Antigen
Agglutination Tests
Agglutinins
Calcium
Cations
Cell-Free System
Disaccharides
Edetic Acid, Calcium, Sodium Salt
Erythrocytes
Hydrogen-Ion Concentration

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