An effective biphase system accelerates hesperidinase-catalyzed conversion of rutin to isoquercitrin

Scientific Reports
Jun WangFu-An Wu

Abstract

Isoquercitrin is a rare, natural ingredient with several biological activities that is a key precursor for the synthesis of enzymatically modified isoquercitrin (EMIQ). The enzymatic production of isoquercitrin from rutin catalyzed by hesperidinase is feasible; however, the bioprocess is hindered by low substrate concentration and a long reaction time. Thus, a novel biphase system consisting of [Bmim][BF4]:glycine-sodium hydroxide (pH 9) (10:90, v/v) and glyceryl triacetate (1:1, v/v) was initially established for isoquercitrin production. The biotransformation product was identified using liquid chromatography-mass spectrometry, and the bonding mechanism of the enzyme and substrate was inferred using circular dichroism spectra and kinetic parameters. The highest rutin conversion of 99.5% and isoquercitrin yield of 93.9% were obtained after 3 h. The reaction route is environmentally benign and mild, and the biphase system could be reused. The substrate concentration was increased 2.6-fold, the reaction time was reduced to three tenths the original time. The three-dimensional structure of hesperidinase was changed in the biphase system, which α-helix and random content were reduced and β-sheet content was increased. Thus, the de...Continue Reading

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Citations

Oct 30, 2016·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Abraham NyskaRobert R Maronpot
Jul 25, 2018·International Journal of Molecular Sciences·Kristýna SlámováKateřina Valentová
Oct 28, 2019·Journal of Enzyme Inhibition and Medicinal Chemistry·Elida Paula Dini de FrancoPatrícia de Oliveira Carvalho
Mar 28, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Debabrata MahapatraRobert R Maronpot

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Methods Mentioned

BETA
circular dichroism

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