An effective inactivant based on singlet oxygen-mediated lipid oxidation implicates a new paradigm for broad-spectrum antivirals.

Redox Biology
Lei ZengBei-Bei Chu

Abstract

Emerging viral pathogens cause substantial morbidity and pose a severe threat to health worldwide. However, a universal antiviral strategy for producing safe and immunogenic inactivated vaccines is lacking. Here, we report an antiviral strategy using the novel singlet oxygen (1O2)-generating agent LJ002 to inactivate enveloped viruses and provide effective protection against viral infection. Our results demonstrated that LJ002 efficiently generated 1O2 in solution and living cells. Nevertheless, LJ002 exhibited no signs of acute toxicity in vitro or in vivo. The 1O2 produced by LJ002 oxidized lipids in the viral envelope and consequently destroyed the viral membrane structure, thus inhibiting the viral and cell membrane fusion necessary for infection. Moreover, the 1O2-based inactivated pseudorabies virus (PRV) vaccine had no effect on the content of the viral surface proteins. Immunization of mice with LJ002-inactiviated PRV vaccine harboring comparable antigen induced more neutralizing antibody responses and efficient protection against PRV infection than conventional formalin-inactivated vaccine. Additionally, LJ002 inactivated a broad spectrum of enveloped viruses. Together, our results may provide a new paradigm of using b...Continue Reading

References

Jan 1, 1991·Free Radical Biology & Medicine·H EsterbauerH Zollner
Jan 1, 1989·Chemico-biological Interactions·J R Kanofsky
Nov 1, 1989·Archives of Biochemistry and Biophysics·P Di MascioH Sies
Aug 1, 1973·The Journal of General Virology·S De Flora, G Badolati
Nov 15, 1997·World Journal of Surgery·M G Michaels
Jul 2, 1999·Biophysical Journal·L S ShlyakhtenkoY L Lyubchenko
Aug 3, 2000·Australian Veterinary Journal·S C MacDiarmid
Mar 18, 2004·Antioxidants & Redox Signaling·Albert W Girotti, Tamas Kriska
Jan 22, 2005·Applied Microbiology and Biotechnology·N Bardiya, J H Bae
Jul 13, 2005·Journal of Plant Physiology·Jerzy KrukAchim Trebst
Jul 25, 2006·Nature Medicine·Amin MoghaddamPeter J M Openshaw
Oct 13, 2006·Journal of the American Chemical Society·Bo SongJingli Yuan
Nov 14, 2006·Forensic Science International : Synergy·Marta ConcheiroManuel López-Rivadulla
Sep 28, 2007·Journal of Oleo Science·Naoki WatabeNorimichi Kawashima
Mar 24, 2009·Trends in Plant Science·Christian Triantaphylidès, Michel Havaux
May 14, 2009·Chemical Communications : Chem Comm·Xavier RagàsSanti Nonell
Jun 27, 2009·Phytochemistry·Martin J Mueller, Susanne Berger
Jan 19, 2010·Veterinary Pathology·J ArztC C Brown
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Mike C WolfBenhur Lee
Oct 23, 2010·Journal of Dairy Science·D ScheideggerS C Kivatinitz
Jul 5, 2011·Advances in Microbial Physiology·J GlaeserGabriele Klug
Sep 20, 2011·Current Opinion in Virology·Richard K Plemper
Nov 6, 2013·Emerging Infectious Diseases·Tong-Qing AnGuang-Zhi Tong
Jul 31, 2014·Journal of the American Chemical Society·Sooyeon KimTetsuro Majima
Mar 15, 2015·Molecular Immunology·Jiang WangGuoyu Yang
Jun 16, 2015·Nature Reviews. Microbiology·Frederic VigantBenhur Lee
Mar 19, 2016·Genome Announcements·Shuangshuang XiangKegong Tian
Mar 16, 2017·Expert Review of Vaccines·Sandra ScheiblhoferJosef Thalhamer
Jun 25, 2017·Biologicals : Journal of the International Association of Biological Standardization·Arunava SarkarVeerakyathappa Bhanuprakash
Mar 20, 2018·Advances in Virus Research·Yolanda RevillaJuergen A Richt
May 19, 2018·Emerging Infectious Diseases·Jing-Wen AiWen-Hong Zhang
Aug 14, 2018·Transboundary and Emerging Diseases·Xintao ZhouRongliang Hu
Feb 15, 2019·Nature·Christopher P StanleyRoland Stocker

❮ Previous
Next ❯

Citations

May 29, 2021·Science China. Life Sciences·Guoli LiBeibei Chu
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Kseniya A MariewskayaAlexey V Ustinov
Oct 21, 2021·The FEBS Journal·Salimata Bagayoko, Etienne Meunier
Jan 4, 2022·ACS Applied Materials & Interfaces·Taylor WrightMichael O Wolf

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
Protein Assay
fluorescence microscopy
MDA
electron microscopy
density gradient centrifugation
confocal microscopy
AFM
dot blot
dot-blot

Software Mentioned

ZEN
GraphPad Prism
ImageJ
Prism
Image J

Related Concepts

Related Feeds

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Related Papers

Microbiology and Molecular Biology Reviews : MMBR
Lisa E PomeranzChristoph J Hengartner
Nature Reviews. Microbiology
Frederic VigantBenhur Lee
Expert Review of Anti-infective Therapy
Jae-Min Song, Baik-Lin Seong
Current Opinion in Virology
Richard K Plemper
© 2022 Meta ULC. All rights reserved