An effective method for improving the fertility of glycerol-exposed poultry semen

Poultry Science
J A Long, G Kulkarni

Abstract

Semen cryopreservation is necessary for banking germplasm from critical poultry stocks. To date, glycerol is the most effective cryoprotectant for poultry sperm; however, the contraceptive effects of glycerol require a significant reduction of the cryoprotectant from thawed semen before artificial insemination (AI). The effectiveness of glycerol reduction by dialysis, Percoll density gradient centrifugation, or washing through 12% (wt/vol) Accudenz was evaluated by fertility trials with highly inbred chicken research lines and commercial turkey lines. Semen was extended 1:1 and then diluted with glycerolized extender to yield a final 11% (vol/vol) glycerol concentration. Glycerolized rooster semen was aliquoted for control, Accudenz centrifugation, and dialysis treatments. A total of 90 pure line and 85 F1 hybrid chicken hens were each inseminated with 100 x 10(6) sperm at 7-d intervals for 4 to 6 wk. All eggs from the glycerolized control semen treatments were infertile, and fertility rates from dialyzed semen decreased steadily from 26.4 to 0% within the first 4 wk for the pure lines. In contrast, fertility rates for Accudenz-processed semen increased from 17.9 to 37.17% during the first 4 wk. Similar fertility rates occurred...Continue Reading

Citations

Dec 5, 2014·Reproduction in Domestic Animals = Zuchthygiene·F M K AbouelezzJ Santiago-Moreno
Dec 21, 2005·Theriogenology·Julie A Long, H David Guthrie
Sep 24, 2019·British Poultry Science·H L LinL R Chen
Sep 12, 2017·Reproduction in Domestic Animals = Zuchthygiene·M MirandaP Chrenek
Dec 9, 2017·Annual Review of Animal Biosciences·H D Blackburn
Jan 21, 2021·Animals : an Open Access Journal From MDPI·María Gemma Millán de la BlancaJulián Santiago-Moreno
Oct 2, 2019·Theriogenology·Masoumeh SalehiAbdolhossein Shahverdi

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