An effective methodology for simultaneous quantification of thiophanate-methyl, and its metabolite carbendazim in pear, using LC-MS/MS

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Han Sol LeeJae-Han Shim

Abstract

Herein we have developed an optimized analytical method for the simultaneous quantification of a fungicide, thiophanate-methyl, and its metabolite, carbendazim, in pear cultivated under open-field conditions using liquid chromatography-triple quadrupole mass spectrometry (LC-MS/MS). Due to the problem of partitioning associated with using acetonitrile and salts, methanol was used for sample extraction; the extract was then filtered using a vacuum filter, and cleaned-up using C18 QuEChERS bulk sorbent following dispersive solid-phase extraction (d-SPE) procedure. Since a common problem, "matrix effect", associated with the matrix was observed in LC-MS/MS, calibration curves for both thiophanate-methyl and carbendazim were constructed in the matrix using seven different concentration levels. Excellent linearity was observed, with determinant coefficient (R2) ≥ 0.9990. The limits of quantification (LOQs) were ≤0.02 mg/kg, satisfactory in terms of the maximum residue limits. Methods were validated at two fortification concentrations (10 × LOQ and 50 × LOQ); the experiments were repeated three times for each level and the average recoveries were 75.00-84.92%, with the coefficient of variation (CV) being ≤5.78%. The developed analyti...Continue Reading

Citations

Aug 21, 2021·Food Chemistry·Leonardo ValderramaEduardo Carasek
Oct 17, 2020·Journal of Agricultural and Food Chemistry·Su-Yan WangPedro Laborda
Sep 28, 2021·Critical Reviews in Food Science and Nutrition·Md Mehedi HassanQuansheng Chen

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