PMID: 11908513Mar 23, 2002Paper

An efficient and reproducible method for measuring hydrogen peroxide in exhaled breath condensate

Respiratory Medicine
Wendy J C van BeurdenF W J M Smeenk

Abstract

We investigated the sensitivity and reproducibility of a test procedure for measuring hydrogen peroxide (H202) in exhaled breath condensate and the effect of storage of the condensate on the H2O2 concentration, and compared the results to previous studies. Twenty stable COPD patients breathed into our collecting device twice for a period of 10 min. The total exhaled air volume (EAV) and condensate volume were measured both times and the H2O2 concentration of the condensate was determined fluorimetrically. The concentration was measured again after freezing the reaction product at -70 degrees C for a period of 10, 20 and 40 days. We collected 2-5 ml condensate in 10 min. The EAV and condensate volumes were strongly correlated. There was no significant difference between the mean H2O2 concentration of the first and second test. We obtained a detect on limit for the H2O2 concentration of 0.02 micromoll(-1). The H2O2 concentration appeared to remain stable for a period up to 40 days of freezing. Compared to previous studies we developed a more efficient breath condensate collecting device and obtained a lower H2O2 detection limit. The measurement of exhaled H2O2 was reproducible. In addition, storage of the samples up to 40 days sh...Continue Reading

References

Sep 1, 1996·American Journal of Respiratory and Critical Care Medicine·P N DekhuijzenA Bast
Aug 1, 1997·American Journal of Respiratory and Critical Care Medicine·J E RepineI Lankhorst
Sep 10, 1998·American Journal of Respiratory and Critical Care Medicine·S LoukidesP J Barnes
Jun 3, 2000·The European Respiratory Journal·L P HoA P Greening
Apr 9, 2001·The European Respiratory Journal·M B SchleissR A Jörres

❮ Previous
Next ❯

Citations

Jun 22, 2011·Journal of Aerosol Medicine and Pulmonary Drug Delivery·Reka GajdocsiSergei A Kharitonov
Dec 21, 2002·American Journal of Respiratory and Critical Care Medicine·Géza VassIldikó Horváth
Mar 11, 2003·American Journal of Respiratory Cell and Molecular Biology·Corinne Wijkstrom-FreiGregory E Conner
Feb 22, 2012·Sensors·Alphus D Wilson, Manuela Baietto
Sep 2, 2010·Clinica Chimica Acta; International Journal of Clinical Chemistry·Robert StolarekDariusz Nowak
Jul 6, 2010·Pulmonary Pharmacology & Therapeutics·Z DiamantR Gerth van Wijk
Mar 5, 2004·Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology·Philippe P R RosiasQuirijn Jöbsis
Dec 21, 2004·Pulmonary Pharmacology & Therapeutics·Fernando De BenedettoClaudio M Sanguinetti
Jun 7, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Paolo Montuschi
Aug 26, 2004·Redox Report : Communications in Free Radical Research·Irfan Rahman, Saibal K Biswas
Jan 22, 2015·Journal of Exposure Science & Environmental Epidemiology·Jaime Mirowsky, Terry Gordon
May 16, 2008·Environmental Health : a Global Access Science Source·Michael J EptonLeslie Brown
Apr 22, 2016·Journal of Physiology and Biochemistry·M TuestaO F Araneda
Dec 12, 2012·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Tzu-Chien KaoGow-Chin Yen
Aug 9, 2005·Respiratory Medicine·Wim B GerritsenFred J Haas
Dec 10, 2009·The Journal of Asthma : Official Journal of the Association for the Care of Asthma· RatnawatiPaul S Thomas
Apr 24, 2012·Journal of Breath Research·Philippe Rosias
Jul 27, 2018·Analytical and Bioanalytical Chemistry·Elaheh RahimpourAbolghasem Jouyban
Feb 17, 2009·Jornal brasileiro de pneumologia : publicaça̋o oficial da Sociedade Brasileira de Pneumologia e Tisilogia·Oscar Florencio Araneda Valenzuela, Maria Paulina Salazar Encina
Sep 4, 2021·Expert Review of Proteomics·Natalia ZakharovaAlexey S Kononikhin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

The Journal of Asthma : Official Journal of the Association for the Care of Asthma
Amina Hamed Ahmad Al Obaidi, Abdulghani Mohamad Al Samarai
© 2022 Meta ULC. All rights reserved