An efficient method for miRNA detection and localization in crop plants

Frontiers in Plant Science
Flor de Fátima Rosas-CárdenasStefan de Folter


microRNAs are a class of non-coding small RNAs (sRNAs) that are important regulators of gene expression at the post-transcriptional level by mRNA cleavage or translation inhibition. Another class of sRNAs are siRNAs, which also regulate gene expression but by causing DNA methylation. This epigenetic regulatory role has been observed for some miRNAs as well. The use of sRNAs allows the development of biotechnological applications in plants. To develop these types of applications, and to better understand the natural roles they play, it is important to be able to detect and to localize these sRNAs at the plant tissue level. Sometimes, in crop plants this can be challenging. Therefore, we developed a tissue printing hybridization protocol for easy and efficient detection of sRNAs and demonstrate this by the analysis of the spatio-temporal expression patterns of the miRNAs miR159 and miR164 in fruits of various crop plants. Moreover, we show the possibility to also detect the expression of miRNAs in fruit juice using a dot blot hybridization approach.


Apr 1, 1994·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J E Varner, Z Ye
Aug 12, 2000·Plant Physiology·N HollandD P Delmer
Nov 30, 2005·Nucleic Acids Research·Caifu ChenKarl J Guegler
Sep 8, 2006·Methods in Molecular Biology·Catherine Kidner, Marja Timmermans
Jan 15, 2008·Trends in Biotechnology·James Q YinKevin V Morris
Jun 10, 2008·Nature Protocols·Gurman S Pall, Andrew J Hamilton
Feb 26, 2009·Cell·Olivier Voinnet
Apr 13, 2010·Journal of Agricultural and Food Chemistry·Ruben P JolieMarc E Hendrickx
Jan 22, 2011·Circulation Research·Eva van Rooij
Feb 26, 2011·Plant Methods·Flor de Fátima Rosas-CárdenasStefan de Folter
Mar 25, 2011·Methods in Molecular Biology·Eva Várallyay, Zoltán Havelda
Sep 8, 2011·Methods in Molecular Biology·Wioletta E PluskotaHiro Nonogaki
Dec 4, 2012·Plant Physiology·Pablo A ManavellaDetlef Weigel
Sep 24, 2013·Journal of Virological Methods·Filipa EstevesFilomena Fonseca
Apr 25, 2014·Nature Communications·Ricardo A Chávez MontesStefan de Folter
Oct 8, 2014·Methods in Molecular Biology·Tomofumi Mochizuki, Satoshi T Ohki
Nov 5, 2014·Planta·Flor de Fátima Rosas-CárdenasStefan de Folter

Related Concepts

MRNA Cleavage
Establishment and Maintenance of Localization
microRNA-164, Arabidopsis
Fruit Juices
Small Nuclear RNA
DNA Methylation [PE]
Crops, Agricultural
RNA, Small Interfering
Nucleic Acid Hybridization Procedure
MIRN159 microRNA, Arabidopsis

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