PMID: 11324726Apr 28, 2001Paper

An efficient method for the culture of Helicobacter pylori from gastric biopsies with two-section petri dishes

Journal of Gastroenterology
H J DingC Y Chen

Abstract

Helicobacter pylori is the principal cause of chronic gastritis and is strongly associated with peptic ulcer disease and gastric cancer, as well as with gastric lymphoma (mucosa-associated lymphoid tissue [MALT] type). H. pylori could be recovered by many kinds of media, however, it could not be detected efficiently. This study was trying to ameliorate the isolation and detection rate with selective and non-selective media. First, type strain (ATCC 43504) was cultured with twelve different kinds of sheep blood agar based on brain heart infusion medium for five days. Then five better non-selective media and five selective media were chosen for the biopsy, which were incubated at 37 degrees C in a microaerophilic incubator for five days. Endoscopic biopsies were collected from 623 patients at Kaohsiung Medical University Hospital. It was found that non-selective media, which contain 5% modified chocolate agar (the ratio of fresh and heated sheep blood is 2) with 1% Isovitalex, had the best detection rate (91.1 +/- 0.6)%. Furthermore, 5% modified chocolate agar with 1% Isovitalex and 1% antibiotics would increase the detection rate to (97.1 +/- 0.3)%. According to Ansorg's method, the best detection rate (98.4 +/- 0.7)% could be o...Continue Reading

Citations

Jan 22, 2011·International Journal of Dentistry·Masahiro YonedaTakao Hirofuji
Mar 28, 2013·International Journal of Dentistry·N SuzukiT Hirofuji
Jul 14, 2010·Journal of Microbiological Methods·Israel Castillo-JuarezIrma Romero

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