An efficient Oligo-FISH painting system for revealing chromosome rearrangements and polyploidization in Triticeae.

The Plant Journal : for Cell and Molecular Biology
Guangrong LiZujun Yang

Abstract

A chromosome-specific painting technique has been developed which combines the most recent approaches of the companion disciplines of molecular cytogenetics and genome research. We developed seven oligonucleotide (oligo) pools derivd from single-copy sequences on chromosomes 1 to 7 of barley (Hordeum vulgare L.) and corresponding collinear regions of wheat (Triticum aestivum L.). The seven groups of pooled oligos comprised between 10 986 and 12 496 45-bp monomers, and these then produced stable fluorescence in situ hybridization (FISH) signals on chromosomes of each linkage group of wheat and barley. The pooled oligo probes were applied to high-throughput karyotyping of the chromosomes of other Triticeae species in the genera Secale, Aegilops, Thinopyrum, and Dasypyrum, and the study also extended to some wheat-alien amphiploids and derived lines. We demonstrated that a complete set of whole-chromosome oligo painting probes facilitated the study of inter-species chromosome homologous relationships and visualized non-homologous chromosomal rearrangements in Triticeae species and some wheat-alien species derivatives. When combined with other non-denaturing FISH procedures using tandem-repeat oligos, the newly developed oligo pain...Continue Reading

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Citations

Jan 30, 2021·Genome Génome / Conseil National De Recherches Canada·Zhihui YuZujun Yang
Jun 22, 2021·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Manman QuYonghua Han
Aug 7, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Yangyang SunLihui Li
Aug 10, 2021·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Fernanda de Oliveira BustamanteAna Christina Brasileiro-Vidal

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