An efficient one-pot four-segment condensation method for protein chemical synthesis

Angewandte Chemie
Shan TangLei Liu

Abstract

Successive peptide ligation using a one-pot method can improve the efficiency of protein chemical synthesis. Although one-pot three-segment ligation has enjoyed widespread application, a robust method for one-pot four-segment ligation had to date remained undeveloped. Herein we report a new one-pot multisegment peptide ligation method that can be used to condense up to four segments with operational simplicity and high efficiency. Its practicality is demonstrated by the one-pot four-segment synthesis of a plant protein, crambin, and a human chemokine, hCCL21.

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Citations

Jan 13, 2016·Organic Letters·Keisuke AiharaAkira Otaka
Mar 19, 2016·Journal of Peptide Science : an Official Publication of the European Peptide Society·Huan LanLei Liu
May 27, 2016·Nature Protocols·Thibault J HarmandJeffrey W Bode
Aug 2, 2016·Journal of the American Chemical Society·Chi Lung LeeXuechen Li
Dec 10, 2016·The Journal of Organic Chemistry·Arumugam VigneshNallasamy Dharmaraj
Apr 15, 2016·Chemistry : a European Journal·Yi-Chao HuangYi-Ming Li
Apr 23, 2016·Organic & Biomolecular Chemistry·Yun-Kun QiLei Liu
May 18, 2016·Organic & Biomolecular Chemistry·Chao ZuoJi-Shen Zheng
Sep 1, 2017·Chemical Communications : Chem Comm·Xiao-Dan TanYi-Ming Li
May 29, 2018·Organic & Biomolecular Chemistry·Kiran BajajGirinath G Pillai
Jun 9, 2018·Chemical Communications : Chem Comm·Kohei SatoNobuyuki Mase
Jul 17, 2018·Journal of Peptide Science : an Official Publication of the European Peptide Society·Qian QuYi-Ming Li
Mar 10, 2019·Protein Science : a Publication of the Protein Society·Jean A BoutinHubert Vaudry
Oct 4, 2019·Chemical Communications : Chem Comm·Jun LiangJia-Bin Li
Jul 3, 2019·Chembiochem : a European Journal of Chemical Biology·Jun LiuSharon Rozovsky
Aug 12, 2020·Chemistry, an Asian Journal·Yun-Kun QiLei Liu
Feb 24, 2018·Frontiers in Chemistry·Yun-Kun QiBaihui Yan
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Sep 2, 2021·Journal of Peptide Science : an Official Publication of the European Peptide Society·Yu-Lei LiGe-Min Fang
Nov 24, 2021·Journal of Peptide Science : an Official Publication of the European Peptide Society·Huasong AiJia-Bin Li

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