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An efficient one-step site-directed and site-saturation mutagenesis protocol

Nucleic Acids Research

Aug 12, 2004

Lei ZhengJean-Louis Reymond

Abstract

We have developed a new primer design method based on the QuickChange site-directed mutagenesis protocol, which significantly improves the PCR amplification efficiency. This design method minimizes primer dimerization and ensures the priority of primer-template annealing over primer sel...read more

Mentioned in this Paper

Electrophoresis, Agar Gel
Mutagenesis, Site-Directed
One-Step Dentin Bonding System
Gene Amplification Technique
In Vitro Molecular Evolution
Codon (Nucleotide Sequence)
Oligonucleotide Primers
Mutagenesis Procedure
cDNA Library
Gene Amplification Abnormality
1176
1
1
Paper Details
References
  • References8
  • Citations453
1
  • References8
  • Citations453
12345...

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An efficient one-step site-directed and site-saturation mutagenesis protocol

Nucleic Acids Research

Aug 12, 2004

Lei ZhengJean-Louis Reymond

PMID: 15304544

DOI: 10.1093/nar/gnh110

Abstract

We have developed a new primer design method based on the QuickChange site-directed mutagenesis protocol, which significantly improves the PCR amplification efficiency. This design method minimizes primer dimerization and ensures the priority of primer-template annealing over primer sel...read more

Mentioned in this Paper

Electrophoresis, Agar Gel
Mutagenesis, Site-Directed
One-Step Dentin Bonding System
Gene Amplification Technique
In Vitro Molecular Evolution
Codon (Nucleotide Sequence)
Oligonucleotide Primers
Mutagenesis Procedure
cDNA Library
Gene Amplification Abnormality
1176
1
1

Feeds With Similar Papers

Structure and Function of DNA

The double helix structure of DNA is important for its biological function. Discover the latest research on the Structure and Function of DNA here.

Microbial ecology, genetics and evolution

Microbes are microscopic organisms which may exist in a unicellular or colony form. Here is the latest research on their genetic makeup, their relationship with one another and the environment, and their evolution.

Related Papers

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A novel megaprimed and ligase-free, PCR-based, site-directed mutagenesis method

Analytical BiochemistryJanuary 17, 2008
Wen-Chi TsengTsuei-Yun Fang
Paper Details
References
  • References8
  • Citations453
1
  • References8
  • Citations453
12345...

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