PMID: 9530827Apr 8, 1998Paper

An efficient procedure for studying pectin structure which combines limited depolymerization and 13C NMR

European Biophysics Journal : EBJ
L CatoireC Hervé du Penhoat

Abstract

A protocol for partial thermally-induced depolymerization of differently methoxylated pectin samples is described. The resulting macromolecules have been fully characterized with various complementary techniques, such as size exclusion chromatography (SEC), potentiometry, viscometry and 13C NMR. Optimum conditions afford samples at 50-80% yield with weight-average molecular weights in the 4 to 20 kDa range. The major fraction of these polysaccharides adopts the random-coil conformation and such samples are suitable for 13C NMR structural studies at room temperature. The methoxyl distributions of two apple pectin samples with a degree of esterification (DE) between 54 and 74% and a citrus pectin (DE, 72%) were shown to be random in nature, whereas that of a lightly methoxylated apple pectin (DE 39%) was partially blockwise. The carbon relaxation parameters of the depolymerized pectins attain asymptotic values for Mw > 4 kDa. The Mw values estimated from intrinsic viscosity data with the Mark-Houwink relationship reported for native pectins are in good agreement with those obtained by either end-group analysis (NMR) or SEC. Thus, all the physicochemical data indicate that the secondary structure of the isolated chains of depolyme...Continue Reading

Citations

Dec 31, 2005·Journal of Industrial Microbiology & Biotechnology·Mondher Th Numan, Narayan B Bhosle
May 3, 2002·Phytochemistry·Victoria V GolovchenkoYury S Ovodov
Jul 20, 2010·Biochemistry. Biokhimii︠a︡·F V VityazevYu S Ovodov
Jan 14, 2009·The European Physical Journal. E, Soft Matter·R R VincentM A K Williams
Aug 2, 2007·Chemical & Pharmaceutical Bulletin·Chikako TakedaYoshiharu Machida
Mar 8, 2008·Bioscience, Biotechnology, and Biochemistry·Yukihiro TamakiMasakuni Tako
Apr 30, 2013·International Journal of Biological Macromolecules·Xuehong ZhangCuiwu Lin
Mar 8, 2013·Carbohydrate Polymers·Zuzana KošťálováAnna Ebringerová
May 11, 2016·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Andrea PensadoAlejandro Sanchez
Jul 26, 2006·Molecular Nutrition & Food Research·Bjørge WesterengAnne Berit Samuelsen
Oct 31, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Raquel Teixeira Terceiro PaimMaria Izabel Florindo Guedes
Jul 30, 2014·Biochimica Et Biophysica Acta·Alexandre A ArnoldIsabelle Marcotte
Jan 4, 2012·Carbohydrate Polymers·Jingjing LiYifa Zhou

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cajal Bodies & Gems

Cajal bodies or coiled bodies are dense foci of coilin protein. Gemini of Cajal bodies, or gems, are microscopically similar to Cajal bodies. It is believed that Cajal bodies play important roles in RNA processing while gems assist the Cajal bodies. Find the latest research on Cajal bodies and gems here.