An efficient strategy to assemble water soluble histidine-perylene diimide and graphene oxide for the detection of PPi in physiological conditions and in vitro

Biosensors & Bioelectronics
B MuthurajParameswar K Iyer

Abstract

A strategy to develop water soluble, biocompatible nanocomposite probe for the detection of pyrophosphate (PPi) in physiological conditions and in in vitro live melanoma cancer cells (B16F10) is reported. The self-assembled nanocomposite probe comprised of amino acid (histidine) functionalized perylenediimide (PDI-HIS), copper ion and graphene oxide (GO) and that could be utilized as a highly effective sensing platform in biological conditions and cellular environment via fluorescence "turn-on" for PPi detection. This controlled fabrication of metal organic self-assembled spheres along with GO proved very valuable for the detection of PPi in unprecedented sensitivity over other competing ions. The PDI-HIS-Cu-GO (PCG) nanocomposite sensor provides a unique platform for the fluorogenic detection of PPi having a very low limit of detection (LOD) of 0.60×10-7M based on the strong affinity (1.0×106M-1) between the copper complex of PDI-HIS receptor and PPi. The intracellular detection of PPi using PCG also carried out in B16F10 cells where >10 times observed as compared to the PDI-HIS+Cu2+ complex. Thus early cancer detection via PPi recognition in physiological conditions and in live cells was possible using PCG. Furthermore, the f...Continue Reading

References

Aug 15, 1998·Science·M RonaghiP Nyrén
Nov 13, 2003·Chemical Reviews·Ramón Martínez-Máñez, Félix Sancenón
Oct 23, 2004·Science·K S NovoselovA A Firsov
Jun 23, 2009·Science·A K Geim
Aug 25, 2010·ACS Nano·Daniela C MarcanoJames M Tour
Dec 7, 2011·Chemical Society Reviews·Yuxin LiuPeng Chen
May 26, 2012·Advanced Materials·Eden Morales-Narváez, Arben Merkoçi
Nov 20, 2013·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Xuzhi ZhuKirk S Schanze
Dec 18, 2013·Chemical Society Reviews·Jing-Juan XuHong-Yuan Chen
Feb 8, 2014·Theranostics·Sudip MukherjeeChitta Ranjan Patra
Apr 8, 2014·ACS Applied Materials & Interfaces·Balakrishnan MuthurajParameswar Krishnan Iyer
Dec 23, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Junfeng WangYi Pang
Jan 13, 2015·Chemical Society Reviews·Songyi LeeJuyoung Yoon
Sep 5, 2015·ACS Applied Materials & Interfaces·Balakrishnan MuthurajParameswar K Iyer

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Citations

Nov 15, 2017·Future Science OA·Sudip MukherjeeChitta Ranjan Patra
May 17, 2019·Biomaterials Science·Susheel Kumar NethiSudip Mukherjee
Jul 27, 2021·ACS Biomaterials Science & Engineering·Hyesung LeeSang-Yup Lee

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