PMID: 2504279May 16, 1989Paper

An elastase-dependent pathway of plasminogen activation

Biochemistry
R Machovich, W G Owen

Abstract

In reaction mixtures containing Glu-plasminogen, alpha 2-antiplasmin, and tissue plasminogen activator or urokinase, either pancreatic or leukocyte elastase enhances the rate of plasminogen activation by 2 or more orders of magnitude. This effect is the consequence of several reactions. (a) In concentrations on the order of 100 nM, elastase degrades plasminogen within 10 min to yield des-kringle1-4-plasminogen (mini-plasminogen), which is 10-fold more efficient than Glu-plasminogen as a substrate for plasminogen activators. Des-kringle1-4-plasminogen is insensitive to cofactor activities of fibrin(ogen) fragments or an endothelial cell cofactor. (b) Des-kringle1-4-plasmin is one-tenth as sensitive as plasmin to inhibition by alpha 2-antiplasmin: k" = 10(6) M-1 s-1 versus 10(7) M-1 s-1. (c) alpha 2-Antiplasmin is disabled efficiently by elastase, with a k" of 20,000 M-1 s-1. The elastase-dependent reactions are not influenced by 6-aminohexanoate. In diluted (10-fold) blood plasma, the capacity of endogenous inhibitors to block plasmin expression is suppressed by 30 microM elastase. It is proposed that elastases provide an alternative pathway for Glu-plasminogen activation and a mechanism for controlling initiation of fibrinolysi...Continue Reading

References

Apr 12, 1979·Biochimica Et Biophysica Acta·U ChristensenI Clemmensen
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Citations

Oct 15, 1995·Thrombosis Research·E T Bach-GansmoO H Skjønsberg
Aug 1, 1990·Biochimica Et Biophysica Acta·R Machovich, W G Owen
Mar 15, 1995·Biochimica Et Biophysica Acta·K KolevR Machovich
Dec 13, 2000·Thrombosis Research·G VörösR Machovich
Jun 15, 1997·Thrombosis Research·C DuboscqL C Kordich
Sep 10, 1994·Annals of the New York Academy of Sciences·A VaheriJ Bizik
Jan 1, 1991·European Journal of Applied Physiology and Occupational Physiology·J B HansenB Osterud
Apr 21, 1997·FEBS Letters·R MachovichW G Owen
Aug 1, 1991·Kidney International·W H Baricos, S V Shah
Apr 3, 2007·Neoplasia : an International Journal for Oncology Research·Miwa AkizukiJun-ichi Yamashita
Jun 5, 2004·Protein Expression and Purification·Weon-Kyoo YouKwang-Hoe Chung
Oct 30, 1990·Biochemical and Biophysical Research Communications·A J GrantD Raghavan
Aug 15, 1997·Archives of Biochemistry and Biophysics·R Machovich, W G Owen
Jun 1, 1992·The British Journal of Surgery·R A LordJ L Francis
Jun 11, 1998·Circulation Research·E KomorowiczR Machovich
Nov 5, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Dayana Barbosa da CruzEduardo Anglés-Cano
Aug 16, 2019·Thrombosis Research·Imre Varjú, Krasimir Kolev
Sep 24, 2005·Journal of Cell Science·Vladimír LeksaHannes Stockinger

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