An electrochemical aptasensor based on hybridization chain reaction with enzyme-signal amplification for interferon-gamma detection

Biosensors & Bioelectronics
Jingjin ZhaoRuqin Yu

Abstract

A novel electrochemical aptasensor based on hybridization chain reaction (HCR) with enzyme-signal amplification was constructed for the detection of interferon-gamma (IFN-γ). In this aptasensor, the recognition probes which contained the sequence of IFN-γ aptamer were initially binded to IFN-γ, and the unbound recognition probes were captured on the electrode as an initiator to trigger the HCR. The two DNA hairpins bio-H1 and bio-H2 were opened by the recognition probe, and bound one by one on the electrode. The biotin was used as a tracer in the hairpins and streptavidin-alkaline phosphatase (SA-ALP) as a reporter molecule. Then, SA-ALP converted its electro-inactive substrate 1-naphthyl phosphate into an electroactive derivative 1-naphthol generating amplified electrochemical signal by differential pulse voltammetry (DPV). The activity of the immobilized enzyme was voltammetrically determined by measuring the amount of 1-naphthol generated for enzymatic dephosphorylation of 1-naphthyl phosphate. The electrochemical signal observed was inversely related to the concentration of IFN-γ. The proposed approach showed a high sensitivity for IFN-γ in a concentration range of 0.5-300 nM with a detection limit of 0.3 nM. The sensing sy...Continue Reading

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Citations

Dec 15, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Yaru ZhangXiang Cai
Nov 10, 2015·Chemical Reviews·Yongxi ZhaoChunhai Fan
Nov 26, 2015·IEEE Transactions on Nanobioscience·Souvik MukherjeeMichael Stroscio
Apr 29, 2015·Biosensors & Bioelectronics·Sidra FaridMichael A Stroscio
Aug 2, 2016·Biosensors & Bioelectronics·Despina MoschouThemistoklis Prodromakis
Sep 26, 2019·Critical Reviews in Biotechnology·Behrouz GolichenariJavad Behravan
Mar 14, 2020·Chemical Communications : Chem Comm·Xing LuLei Li
Mar 21, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jiahao HuangZhigang Li
Aug 9, 2021·Talanta·Jingting WuZai-Sheng Wu
Feb 5, 2019·Chemical Reviews·Friedrich C SimmelHari R Singh
May 8, 2018·ACS Sensors·Erik E AugspurgerMehmet V Yigit

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