An electrochemical sensor based on label-free functional allosteric molecular beacons for detection target DNA/miRNA

Biosensors & Bioelectronics
Zhimin CaiXi Chen

Abstract

We report two novel electrochemical sensors (E-sensors) for the detection of target DNA and miRNA. The E-sensors were fabricated using label-free functional allosteric molecular beacons (aMBs), which can form streptavidin aptamers to bind to streptavidin peroxidase polymer and so generate catalytic currents in the presence of the targets. These E-sensors eliminate the antigen antibody interactions which require sophisticated DNA modification. During the experiment, we found a pair of CV peaks located at around 0.17 V. These peaks contributed to the redox reaction between TMB and TMB(+), and the adsorption-desorption process of TMB(+) to the negative aMB backbone. When the E-sensor was hybridized with the complement of the aMB sequence, a pair of CV peaks were found at around 0.47 V which were related to the redox reaction between TMB(+) and TMB(2+), and the process of intercalation of the planar structure of TMB(2+) to dsDNA. The RSV-aMB E-sensor could detect 44 amol RSV DNA in the 4 μL sample and performed well in complicated biological environments. The let-7a-aMB E-sensor reached a detection limit of 13.6 amol let-7a miRNA in the 4 μL sample and showed good selectivity for one base mismatched miRNA.

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Citations

Dec 15, 2015·Analytical Chemistry·Richard M Graybill, Ryan C Bailey
Jan 7, 2015·Biosensors & Bioelectronics·Mahmoud Labib, Maxim V Berezovski
Dec 24, 2014·Bioanalysis·Ilaria Palchetti
Feb 18, 2014·Chemical Communications : Chem Comm·Jinbo ZhuErkang Wang
Jun 4, 2014·Biosensors & Bioelectronics·Li JiangJiong Li
Oct 31, 2018·The Analyst·Philip GillespieDanny O'Hare
Feb 23, 2017·Veterinary Research·Jasmina VidicNicole Jaffrezic-Renault
Feb 7, 2014·The Analyst·Blake N Johnson, Raj Mutharasan

Related Concepts

Conductometry
DNA, Double-Stranded
Medical Device Design
Poly(A) Tail
Staining and Labeling
Molecular Probe Techniques
Biosensing Techniques
Prosthesis Failure Analysis
Aptamers, Nucleotide
Amphotericin B

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