An electrochemical sensor for pesticide assays based on carbon nanotube-enhanced acetycholinesterase activity

The Analyst
Haode ChenChunhai Fan

Abstract

There has been urgent demand for rapid, sensitive and cost-effective pesticide assay technologies due to the global attention of environmental and food-safety problems. Acetycholinesterase (AChE)-based electrochemical sensors have attracted significant interest toward this goal. In this contribution, we introduced multiwalled carbon nanotubes (MWNTs) into our sensor design, where they played dual enhancement roles; first is that MWNTs loaded on glassy carbon (GC) electrodes significantly increase surface areas, facilitating the electrochemical polymerization of prussian blue (PB), a redox mediator for the electrochemical oxidation of the enzymatic product, thiocholine (TCh). Second, MWNTs enhance the enzymatic activity of AChE, as manifested by the decreased Michaelis-Menten constant (K(m)). As a result of these two important enhancement factors offered by MWNTs, our electrochemical pesticide sensor exhibited rapid response and high sensitivity toward the detection of a series of pesticides. Moreover, we demonstrated that this sensor was stable, reproducible and selective enough for detection in real samples.

References

Jul 1, 1990·Environmental Health Perspectives·T R Fukuto
Dec 18, 2002·Biosensors & Bioelectronics·S SotiropoulouN A Chaniotakis
Jul 1, 1961·Biochemical Pharmacology·G L ELLMANR M FEATHER-STONE
Nov 13, 2003·Journal of Agricultural and Food Chemistry·Beatriz AlberoJosé L Tadeo
Apr 13, 2006·Journal of the American Chemical Society·Sandra Bencic-NagaleDavid R Walt
Aug 28, 2007·International Journal of Nanomedicine·Norah O'FarrellBenjamin R Horrocks

❮ Previous
Next ❯

Citations

Aug 16, 2012·Analytical and Bioanalytical Chemistry·Shaoqin LiuXinyu Li
Aug 18, 2012·Chemical Reviews·Gemma AragayArben Merkoçi
Jan 16, 2014·Journal of Food Science and Technology·R Kalpana SastryN H Rao
Feb 14, 2009·Chemical Communications : Chem Comm·Jiawang Ding, Wei Qin
Sep 20, 2019·Nanomaterials·Mingfei PanShuo Wang
Sep 30, 2016·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Nian Chun GongShuang Yan Huan
Jan 22, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Ming WangDaoben Zhu
Nov 29, 2020·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Baybars KöksoyErhan Demirbas
May 13, 2021·Chemical Communications : Chem Comm·Jianyong LiChunhai Fan
Sep 19, 2018·Chemical Reviews·Vera SchroederTimothy M Swager
Oct 15, 2020·Chemical Reviews·Hui ChenShelley D Minteer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.