An electrochemiluminescence assay for sensitive detection of methyltransferase activity in different cancer cells by hybridization chain reaction coupled with a G-quadruplex/hemin DNAzyme biosensing strategy

The Analyst
Hui ZhangChenxin Cai

Abstract

In this work, a highly sensitive electrochemiluminescence (ECL) assay was fabricated for the detection of human DNA (cytosine-5)-methyltransferase1 (DNMT1) activity in cancer cells. The ECL assay coupled hybridization chain reaction with a G-quadruplex/hemin DNAzyme biosensing strategy. The ECL intensity changes (ΔI) allowed detection of DNMT1 activity down to 0.09 U mL-1, and ΔI was proportional to the logarithm of the activity of DNMT1 within the range of 1.0 to 30.0 U mL-1 in buffer solution. It also showed high sensitivity to DNMT1 activity in A549 cells, with a detection limit of about 2 cells. This ECL assay provides a promising platform for profiling of the mutational cells of tumors and shows a great potential for application to DNA methylation-related clinical diagnostics.

References

Nov 30, 1992·FEBS Letters·P Renbaum, A Razin
Dec 9, 1998·Analytical Chemistry·A B SteelM J Tarlov
Aug 11, 2001·Science·P A Jones, D Takai
Oct 3, 2002·Biochimica Et Biophysica Acta·Yuka YanagisawaKazuo Maruyama
Sep 2, 2005·Nature Reviews. Genetics·Keith D Robertson
Feb 28, 2006·Nucleic Acids Research·Rachna GoyalAlbert Jeltsch
Nov 7, 2008·Methods in Molecular Biology·Yingying ZhangAlbert Jeltsch
May 6, 2010·Analytical Chemistry·Gilad PelossofItamar Willner
Jan 22, 2011·Chemical Communications : Chem Comm·Shuna LiuChenxin Cai
Sep 14, 2011·Methods in Molecular Biology·Renata Z JurkowskaAlbert Jeltsch
Oct 30, 2013·Journal of the American Chemical Society·Natalie B Muren, Jacqueline K Barton
Oct 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Ariel L FurstJacqueline K Barton
Jun 30, 2015·Chemistry & Biology·Ariel L Furst, Jacqueline K Barton
Jun 18, 2016·Analytical and Bioanalytical Chemistry·Huimin DengZhiqiang Gao

❮ Previous
Next ❯

Citations

Mar 20, 2019·Luminescence : the Journal of Biological and Chemical Luminescence·Jiajia DongRunping Han
Sep 5, 2019·Current Medicinal Chemistry·Shu ZhangJunsong Zheng

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biosensors for Cancer Detection

Biosensors are devices that are designed to detect a specific biological analyte by essentially converting a biological entity (ie, protein, DNA, RNA) into an electrical signal that can be detected and analyzed. The use of biosensors in cancer detection and monitoring holds vast potential. Biosensors can be designed to detect emerging cancer biomarkers and to determine drug effectiveness at various target sites. Biosensor technology has the potential to provide fast and accurate detection, reliable imaging of cancer cells, and monitoring of angiogenesis and cancer metastasis, and the ability to determine the effectiveness of anticancer chemotherapy agents.