PMID: 13549501May 25, 1958Paper

An electron microscope study of basophile substances of frozen-dried rat liver

The Journal of Biophysical and Biochemical Cytology
H FINCK

Abstract

Small pieces of liver from rats subjected to different dietary regimes were fixed by freeze-drying, and postfixed by in vacuo heating and denaturation with alcohol. Specimens were digested with ribo- or deoxyribonuclease, and stained with gallocyanin-chromalum, azure II, the Feulgen procedure or alcoholic platinic tetrabromide. Some specimens were reserved as controls of the effects of enzyme treatment. Stained and unstained specimens were embedded in methacrylate and examined by light and electron microscopy. Basophilic and Feulgen-positive substances, after contact with watery reagents, were found by electron microscopy to exist as small dense granules embedded in a less dense homogeneous matrix, forming the walls of submicroscopic vacuoles. These granules were absent after digestion with nucleodepolymerases. In specimens (unstained, or stained with platinic tetrabromide) which had not passed through water, the dense (basophile) substances in nuclei and cytoplasm were found to exist, not as granules, but as ill defined submicroscopic concentrates which blended imperceptibly into the homogeneous matrix of the vacuolar walls. Objections to the use of stains for improving contrast conditions in electron microscopy of tissues are...Continue Reading

References

Sep 1, 1954·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K R PORTER
Sep 1, 1954·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A E MIRSKYM M DALY
Mar 25, 1956·The Journal of Biophysical and Biochemical Cytology·G E PALADE, P SIEKEVITZ
Oct 1, 1951·Stain Technology·R KORSON

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Citations

Dec 1, 1960·The Journal of Biophysical and Biochemical Cytology·S SENO, K YOSHIZAWA
Apr 1, 1961·The Journal of Biophysical and Biochemical Cytology·L I REBHUN
Jan 1, 1980·Progress in Histochemistry and Cytochemistry·G Moyne
Aug 1, 1959·Experimental Cell Research·W H CHASE

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