PMID: 5914697Feb 1, 1966

An electron microscope study of canine cardiac myosin and some of its aggregates

The Journal of Cell Biology
J A Carney, A L Brown

Abstract

The morphology of the canine cardiac myosin molecule has been investigated in the electron microscope with Hall's mica-replica technique. The molecule is an elongated rod (shaft) of nonuniform diameter with a globular expansion (head) on one end. Statistical analysis of the lengths of 1908 molecules showed that the mean length was 1610 +/- 250 A; the mean length of the head was 210 +/- 20 A; and the diameter of the head and that of the shaft were 35 to 40 and 15 to 20 A, respectively. About one-third of the molecules had single or multiple, fairly sharp, angulations along their shafts. Rarely, some details of the substructure of the molecule have been observed. Large, spindle-shaped aggregates, measuring 0.5 to 1 micro in length and 50 to 100 A in diameter, were produced by dilution of the myosin solutions. These aggregates were readily visualized in the electron microscope by means of Huxley's negative-staining technique. Projections often were visible along the length of the aggregates except at a central zone where they were frequently absent. The aggregates resembled the thick myofilaments of the myocardium and appeared similar to those produced by Huxley from skeletal myosin solutions.

References

Oct 1, 1965·Circulation Research·J A Carney, A L Brown
Feb 1, 1953·Archives of Biochemistry and Biophysics·A SZENT-GYORGYI
Oct 11, 1957·Science·A SZENT-GYORGYI, C COHEN
Sep 25, 1957·The Journal of Biophysical and Biochemical Cytology·H E HUXLEY
Sep 1, 1960·The Journal of Clinical Investigation·J O DAVISN A YANKOPOULOS
Jul 1, 1962·Journal of Molecular Biology·J BRAHMS, C M KAY
Jul 1, 1963·Journal of Molecular Biology·C R ZOBEL, F D CARLSON
Jul 1, 1960·The Journal of Biophysical and Biochemical Cytology·C E HALL
Jul 15, 1960·Biochimica Et Biophysica Acta·H NODA, S EBASHI
Sep 30, 1961·Biochimica Et Biophysica Acta·R V RICE
Sep 1, 1951·Biochimica Et Biophysica Acta·B GELOTTE

Citations

Dec 1, 1968·The Journal of Cell Biology·N IkemotoJ Gergely
Jul 1, 1969·The Journal of Cell Biology·D W FAWCETT, N S McNutt
Jul 1, 1969·The Journal of Cell Biology·N S McNutt, D W FAWCETT
Jan 1, 1970·The Journal of Cell Biology·B J Panner, C R Honig
Feb 1, 1972·The Journal of Cell Biology·F A Pepe, B Drucker
Mar 1, 1975·Journal of Microscopy·D G Rayns
Nov 1, 1971·Journal of Ultrastructure Research·O BehnkeL E Nielsen
Sep 1, 1966·Journal of Molecular Biology·B Kaminer, A L Bell
Jun 15, 1973·Journal of Molecular Biology·K Morimoto, W F Harrington

Related Concepts

Canis familiaris
Electron Microscopy
Muscle Proteins
Myocardium
Ultracentrifugation

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