An electron microscopy based method for the detection and quantification of nanomaterial number concentration in environmentally relevant media

The Science of the Total Environment
A PrasadM Baalousha

Abstract

Improved detection and characterization of nanomaterials (NMs) in complex environmental media requires the development of novel sampling approaches to improve the detection limit to be close to environmentally realistic concentrations. Transmission electron microscopy (TEM) is an indispensable metrological tool in nanotechnology and environmental nanoscience due to its high spatial resolution and analytical capabilities when coupled to spectroscopic techniques. However, these capabilities are hampered by the conventional sample preparation methods, which suffer from low NM recovery. The current work presents a validated, fully quantitative sampling technique for TEM that overcomes conventional sample preparation shortcomings, and thus enables the use of TEM for measurement of particle number concentration and their detection in complex media at environmentally realistic concentrations. This sampling method is based on ultracentrifugation of NMs from suspension onto a poly-l-lysine (PLL) functionalized TEM grid, using active deposition (by ultracentrifugation) and retention (by PLL interactions with NM surface) of NMs on the substrate, enabling fully quantitative analysis. Similar analysis with AFM was satisfactory in simple med...Continue Reading

References

Sep 16, 2005·Analytical and Bioanalytical Chemistry·Mohammed BaaloushaPhilippe Le Coustumer
Nov 23, 2007·The Science of the Total Environment·Sabine A BlaserKonrad Hungerbühler
Dec 17, 2008·Environmental Toxicology and Chemistry·Stephen J KlaineJamie R Lead
Dec 17, 2010·Environment International·Julia FabregaJamie R Lead
Apr 14, 2011·Journal of Biomedical Nanotechnology·V BendreA Malloy
May 3, 2011·Journal of Chromatography. a·Isabella RömerJamie R Lead
Oct 25, 2011·Environmental Toxicology and Chemistry·Frank von der KammerJason M Unrine
Mar 1, 2012·Environmental Toxicology and Chemistry·Mohammed BaaloushaJamie R Lead
Mar 1, 2012·Environmental Toxicology and Chemistry·Mohammed BaaloushaJamie R Lead
Jun 19, 2013·Current Opinion in Biotechnology·Ken Donaldson, Craig A Poland
Jul 17, 2013·Environmental Pollution·Fadri GottschalkBernd Nowack

❮ Previous
Next ❯

Citations

Jan 5, 2016·The Science of the Total Environment·Vinayak Laxman PachapurR Y Surampalli
Apr 7, 2016·Environmental Science & Technology·Elijah J PetersenMichael R Winchester
Jun 16, 2017·Environmental Science & Technology·Ruth C MerrifieldJamie R Lead
Apr 11, 2018·Environmental Toxicology and Chemistry·Jamie R LeadKristin Schirmer
Dec 20, 2019·Environmental Science. Nano·Frederic LoosliMohammed Baalousha
Jan 20, 2019·Journal of Colloid and Interface Science·Mithun SikderMohammed Baalousha
Apr 17, 2017·The Science of the Total Environment·Mithun SikderMohammed Baalousha
May 12, 2021·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Ralf KaegiBodo Hattendorf

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.