An electronic, aptamer-based small-molecule sensor for the rapid, label-free detection of cocaine in adulterated samples and biological fluids

Journal of the American Chemical Society
Brian R BakerKevin W Plaxco

Abstract

Whereas spectroscopic and chromatographic techniques for the detection of small organic molecules have achieved impressive results, these methods are generally slow and cumbersome, and thus the development of a general means for the real-time, electronic detection of such targets remains a compelling goal. Here we demonstrate a potentially general, label-free electronic method for the detection of small-molecule targets by building a rapid, reagentless biosensor for the detection of cocaine. The sensor, based on the electrochemical interrogation of a structure-switching aptamer, specifically detects micromolar cocaine in seconds. Because signal generation is based on binding-induced folding, the sensor is highly selective and works directly in blood serum and in the presence of commonly employed interferents and cutting agents, and because all of the sensor components are covalently attached to the electrode surface, the sensor is also reusable: we achieve >99% signal regeneration upon a brief, room temperature aqueous wash. Given recent advances in the generation of highly specific aptamers, this detection platform may be readily adapted for the detection of other small molecules of a wide range of clinically and environmental...Continue Reading

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Citations

Jun 22, 2007·Analytical and Bioanalytical Chemistry·Teresa MairalCiara K O'Sullivan
Sep 25, 2007·Analytical and Bioanalytical Chemistry·Subramanian BalamuruganDavid A Spivak
Oct 24, 2007·Analytical and Bioanalytical Chemistry·Jeong-O LeeYong Hwan Kim
Oct 16, 2008·Analytical and Bioanalytical Chemistry·Zhang ChenLe Deng
Jul 21, 2010·Analytical and Bioanalytical Chemistry·Jing ZhouYong Wang
Nov 1, 2011·Analytical and Bioanalytical Chemistry·Veli Cengiz Ozalp
Jun 23, 2012·Analytical and Bioanalytical Chemistry·Ying LiuAleksandr L Simonian
Nov 29, 2012·Analytical and Bioanalytical Chemistry·Betty C GalarretaFrançois Lagugné-Labarthet
Aug 25, 2009·Bioprocess and Biosystems Engineering·Yoon-Jin KimMan Bock Gu
Sep 21, 2013·Journal of Colloid and Interface Science·Hui JiangJuewen Liu
May 22, 2008·Analytical Chemistry·Benjamin J PrivettMark H Schoenfisch
Mar 21, 2009·Analytical Chemistry·Chun-Yang Zhang, Lawrence W Johnson
Feb 9, 2010·Analytical Chemistry·Lakshmi N CellaAshok Mulchandani
Aug 9, 2011·Accounts of Chemical Research·Michael Famulok, Günter Mayer
Mar 6, 2010·Accounts of Chemical Research·Arica A Lubin, Kevin W Plaxco
Mar 24, 2009·Chemical Reviews·Juewen LiuYi Lu
Jan 12, 2012·Chemical Reviews·Ofer I Wilner, Itamar Willner
Mar 1, 2012·Chemical Reviews·Emil Paleček, Martin Bartošík
Jun 10, 2010·Journal of the American Chemical Society·Fan XiaKevin W Plaxco
Oct 23, 2010·Journal of the American Chemical Society·Takanori UzawaKevin W Plaxco
Feb 9, 2012·Journal of the American Chemical Society·Andrew J BonhamKevin W Plaxco
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Aug 12, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Ryan J WhiteKevin W Plaxco
Apr 16, 2009·Nano Letters·Hansang ChoJeffrey B H Tok

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