An electronic bus bar lies in the core of cytochrome bc1.

Science
Monika SwierczekArtur Osyczka

Abstract

The ubiquinol-cytochrome c oxidoreductases, central to cellular respiration and photosynthesis, are homodimers. High symmetry has frustrated resolution of whether cross-dimer interactions are functionally important. This has resulted in a proliferation of contradictory models. Here, we duplicated and fused cytochrome b subunits, and then broke symmetry by introducing independent mutations into each monomer. Electrons moved freely within and between monomers, crossing an electron-transfer bridge between two hemes in the core of the dimer. This revealed an H-shaped electron-transfer system that distributes electrons between four quinone oxidation-reduction terminals at the corners of the dimer within the millisecond time scale of enzymatic turnover. Free and unregulated distribution of electrons acts like a molecular-scale bus bar, a design often exploited in electronics.

References

Feb 27, 1992·Nature·C C MoserP L Dutton
Aug 31, 2000·Annual Review of Biochemistry·E A BerryA R Crofts
Jul 7, 2001·Trends in Biochemical Sciences·E DarrouzetF Daldal
Oct 9, 2001·Trends in Biochemical Sciences·R J Ellis
Jun 19, 2002·Biochemistry·Florian MullerDavid M Kramer
Feb 13, 2004·Nature·Artur OsyczkaP Leslie Dutton
Jun 8, 2006·Annual Review of Biochemistry·William A CramerJanet L Smith
Jul 29, 2006·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Christopher C MoserP Leslie Dutton
Jan 4, 2007·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Armen Y Mulkidjanian
May 2, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jonathan L CapeDavid M Kramer
Jun 15, 2007·Biochimica Et Biophysica Acta·Haibo ZhangChristopher C Moser
May 13, 2008·Biochimica Et Biophysica Acta·Christopher C MoserP Leslie Dutton
May 13, 2008·Biochimica Et Biophysica Acta·Raul Covian, Bernard L Trumpower
Jun 5, 2008·The Journal of Biological Chemistry·Stefan Dröse, Ulrich Brandt

❮ Previous
Next ❯

Citations

May 11, 2011·Nature Communications·Alizée MalnoëFabrice Rappaport
Jul 17, 2012·Genome Biology and Evolution·Frauke BaymannWolfgang Nitschke
Feb 5, 2014·Biochemistry. Biokhimii︠a︡·V G Grivennikova, A D Vinogradov
Nov 17, 2011·European Biophysics Journal : EBJ·Irene Díaz-MorenoMiguel A De la Rosa
Feb 4, 2014·Plant Physiology and Biochemistry : PPB·Alexander N Tikhonov
Sep 14, 2013·Biochemistry·Bahia Khalfaoui-HassaniFevzi Daldal
Sep 1, 2011·FEBS Letters·Bahia Khalfaoui-HassaniFevzi Daldal
May 29, 2014·The Journal of Physical Chemistry. B·S Saif HasanWilliam A Cramer
Feb 18, 2014·Biochimica Et Biophysica Acta·Michaël L CartronC Neil Hunter
Jul 11, 2012·Chembiochem : a European Journal of Chemical Biology·Wen-Chao YangGuang-Fu Yang
Oct 28, 2015·The Journal of Physical Chemistry. B·Muhammad A HagrasAlexei A Stuchebrukhov
Mar 1, 2011·Biochimica Et Biophysica Acta·William A CramerEiki Yamashita
Jul 23, 2013·Biophysical Journal·Jason N BazilDaniel A Beard
Apr 3, 2012·Biochimica Et Biophysica Acta·Sangjin HongAntony R Crofts
Dec 28, 2012·Biochimica Et Biophysica Acta·Lea Bleier, Stefan Dröse
Nov 6, 2012·Biochimica Et Biophysica Acta·Doreen VictoriaAntony R Crofts
Feb 12, 2013·Biochimica Et Biophysica Acta·Antony R CroftsKlaus Schulten
Jun 1, 2016·Microbiological Research·Beatrycze Nowicka, Jerzy Kruk
May 5, 2012·Biochimica Et Biophysica Acta·Hyung J KimDennis R Winge
Jun 17, 2014·Structure·S Saif Hasan, William A Cramer
Apr 10, 2012·Biochimica Et Biophysica Acta·Monika CzaplaArtur Osyczka
Jul 24, 2013·Biochimica Et Biophysica Acta·Jason W Cooley
Aug 5, 2014·Biochemical and Biophysical Research Communications·Robert EkiertArtur Osyczka
Sep 23, 2016·Nature·James A LettsLeonid A Sazanov
Jan 10, 2017·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Yanfei Zhang, José L Avalos
Feb 14, 2016·Biochimica Et Biophysica Acta·Muhammad A Hagras, Alexei A Stuchebrukhov
May 12, 2017·Physiologia Plantarum·Daria V DibrovaArmen Y Mulkidjanian
Oct 6, 2017·Nature Structural & Molecular Biology·James A Letts, Leonid A Sazanov
Jun 24, 2020·FEBS Letters·Nicholas FisherGiancarlo A Biagini

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.