An electrophysiological study of parthenogenetic activation in mammalian oocytes

Developmental Biology
F Eusebi, G Siracusa

Abstract

Using conventional electrophysiological techniques, we have investigated the electrical responses of mouse and hamster oocytes in metaphase of the second meiotic division to agents which induce parthenogenetic activation. Oocytes from MF1 mice responded to 8.7% ethanol and to 0.3% benzyl alcohol by a depolarization (sometimes preceded by a brief hyperpolarization). The response to ethanol did not "desensitize," and the membrane potential recovered completely when the exposure to ethanol was interrupted. The response was accompanied by a decrease in membrane input resistance (Rin) and had an equilibrium potential of about +5 mV in standard medium and of -10mV in Na-free medium. The oocytes responded to A23187 and to La3+ by an increased Rin, and usually lysed during or after treatment. Multiphasic responses were elicited by ethanol and by Ca-ionophore in metaphase II hamster oocytes; an early hyperpolarization accompanied by a decreased Rin was a common feature of the response to both activating agents. The early hyperpolarization was no longer elicited when the cells were exposed for a second time to ethanol or A23187. K+ and Cl- were the ions mainly involved in the hyperpolarizing potential elicited by A23187, and K+ (but not ...Continue Reading

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Citations

Nov 1, 1991·Molecular Reproduction and Development·G T O'NeillM H Kaufman
Jun 1, 1994·Molecular Reproduction and Development·C TatoneR Colonna
May 1, 1995·Molecular Reproduction and Development·A Bos-MikichD G Whittingham
Jan 5, 2002·Molecular Reproduction and Development·Jin-Yi JiangEimei Sato
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Dec 29, 2011·Proceedings of the National Academy of Sciences of the United States of America·Wei-Min LiuWilliam S B Yeung
Dec 1, 1988·Biological Trace Element Research·T DasG Talukder
Nov 1, 1984·Journal of Cellular Physiology·C CaratschA Salustri

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