An electrospray ionization mass spectrometry investigation of 1-anilino-8-naphthalene-sulfonate (ANS) binding to proteins

Journal of the American Society for Mass Spectrometry
S S RayP Balaram

Abstract

The binding of 1-anilino-8-naphthalene-sulfonic acid (ANS) to various globular proteins at acidic pH has been investigated by electrospray ionization mass spectrometry (ESI-MS). Maximal ANS binding is observed in the pH range 3-5. As many as seven species of dye-bound complexes are detected for myoglobin. Similar studies were carried out with cytochrome c, carbonic anhydrase, triosephosphate isomerase, lysozyme, alpha-lactalbumin, and bovine pancreatic trypsin inhibitor (BPTI). Strong ANS binding was observed wherever molten globule states were postulated in solution. ANS binding is not observed for lysozyme and BPTI, which have tightly folded structures in the native form. Alpha-lactalbumin, which is structurally related to lysozyme but forms a molten globule at acidic pH, exhibited ANS binding. Reduction of disulfide bonds in these proteins leads to the detection of ANS binding even at neutral pH. Binding was suppressed at very low pH (<2.5), presumably due to neutralization of the charge on the sulfonate moiety. The distribution of the relative intensities of the protein bound ANS species varies with the charge state, suggesting heterogeneity of gas phase conformations. The binding strength of these complexes was qualitative...Continue Reading

References

Aug 11, 1982·Biochimica Et Biophysica Acta·J Slavík
Nov 1, 1994·Protein Science : a Publication of the Protein Society·R R LooP C Andrews
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·P G Wolynes
Aug 16, 1994·Biochemistry·Y Konishi, R Feng
Feb 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D SuckauF W McLafferty
Jan 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·K Kuwajima
Jan 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·T E CreightonJ Kemmink
Feb 21, 1997·Journal of Molecular Biology·A FontanaE Scaramella
Feb 1, 1997·Journal of Mass Spectrometry : JMS·D L SmithZ Zhang
Jan 1, 1997·Folding & Design·A FontanaM Zambonin
Jun 1, 1997·Protein Science : a Publication of the Protein Society·E W ChungC V Robinson
Apr 21, 1999·Protein Science : a Publication of the Protein Society·V TsuiP E Wright
Jun 9, 1999·Mass Spectrometry Reviews·S A McLuckey, J L Stephenson
Dec 26, 2001·Chemical Reviews·C S Hoaglund-HyzerD E Clemmer
Aug 1, 1993·Journal of the American Society for Mass Spectrometry·R Feng, Y Konishi

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Citations

Jun 6, 2006·Journal of the American Society for Mass Spectrometry·Theodore A CraigRajiv Kumar
Feb 13, 2003·European Journal of Biochemistry·Priyaranjan PattanaikHemalatha Balaram
Nov 28, 2006·Biochemistry. Biokhimii︠a︡·Aabgeena NaeemRizwan Hasan Khan
Dec 3, 2004·Drug and Chemical Toxicology·Xing HanGary W Jepson
May 4, 2005·Journal of Structural Biology·J Murali, R Jayakumar
Feb 25, 2014·International Journal of Pharmaceutics·Seyed Salman Razavi RohaniMohsen Tafaghodi
Aug 18, 2004·The International Journal of Biochemistry & Cell Biology·Aabgeena Naeem, Rizwan Hasan Khan
Feb 1, 2017·International Journal of Biological Macromolecules·Minhal AbidiAabgeena Naeem
Jul 17, 2012·European Journal of Mass Spectrometry·M Vairamani, S Prabhakar
Aug 28, 2001·Journal of Mass Spectrometry : JMS
Nov 19, 2008·Rapid Communications in Mass Spectrometry : RCM·Carlo Santambrogio, Rita Grandori
Nov 14, 2019·Rapid Communications in Mass Spectrometry : RCM·Ya-Wen LiXian Wang
Dec 11, 2019·Biomolecules·Vladimir N Uversky, Alexei V Finkelstein
Sep 15, 2020·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Carlotta ZoppiLuigi Messori
Nov 3, 2004·Archives of Biochemistry and Biophysics·Aabgeena NaeemRizwan Hasan Khan

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