Abstract
Two chicken single-chain variable antibody fragments (scFv) designated scFv154 and scFv88, previously shown to react with either all or very virulent (vv) infectious bursal disease virus (IBDV) strains, respectively, were evaluated for use in an enzyme-linked immunosorbent assay (ELISA) for differentiation of vvIBDV. Specificity and sensitivity of the vvIBDV ELISA was assessed when scFv154 and scFv88 were expressed as soluble antibodies (sAb), phage antibodies (pAb) or hyper-phage antibodies (hpAb). The highest test sensitivity and specificity was obtained using hpAb154 to detect all IBDV and pAb88 to differentiate vvIBDV strains. Such an ELISA was eight to 16 times more sensitive for IBDV antigen detection than the mouse monoclonal antibody ELISA. Using field samples, the scFv ELISA was able to differentiate between flocks infected with vvIBDV and those infected with classical or variant IBDV. In one instance IBDV was detected in a flock found to be negative by the monoclonal antibody ELISA. The results showed that scFv can be utilized as highly specific and sensitive ELISA reagents for the detection and discrimination of avian pathogens.
References
Jan 1, 1992·Archives of Virology·D B SnyderP K Savage
Aug 11, 1991·Nucleic Acids Research·H R HoogenboomG Winter
May 20, 1988·Science·M BetterA H Horwitz
Mar 1, 1994·The Journal of General Virology·M D BrownM A Skinner
Jul 1, 1997·Analytical Biochemistry·R J KerschbaumerG Himmler
Jan 1, 1997·Archives of Virology·N EterradossiM Guittet
Jul 13, 2000·Archives of Virology·S I Sapats, J Ignjatovic
Aug 17, 2000·Biosensors & Bioelectronics·P A EmanuelA Dion-Schultz
Jan 3, 2001·Nature Biotechnology·S RondotS Dübel
Dec 17, 2002·Current Opinion in Biotechnology·Titus Kretzschmar, Thomas von Rüden
Feb 28, 2003·Archives of Virology·S I SapatsJ Ignjatovic
Aug 21, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Gordana WozniakFlorian Rüker
Oct 3, 2003·Avian Pathology : Journal of the W.V.P.A·L H ParedeJ Ignjatovic
Jul 21, 2004·Journal of Immunological Methods·Andrew R M Bradbury, James D Marks
Nov 17, 2004·Avian Pathology : Journal of the W.V.P.A·T P van den BergH Müller
Jan 15, 2005·Australian Veterinary Journal·J IgnjatovicH Westbury
Jun 1, 2000·Avian Pathology : Journal of the W.V.P.A·T P Berg
Citations
Sep 17, 2010·Expert Review of Molecular Diagnostics·Vinayagamurthy BalamuruganRaj Kumar Singh
Nov 30, 2010·Journal of Virological Methods·Seyed A GhorashiAmir H Noormohammadi
May 16, 2019·Bioengineered·Jiaqi LuoTianrun Liu
Aug 14, 2018·Frontiers in Veterinary Science·Lorena Bustamante-CórdovaJesús Hernández
Jun 7, 2020·Antibodies·Shahbaz Bashir, Jan Paeshuyse