An ELISA for detection of infectious bursal disease virus and differentiation of very virulent strains based on single chain recombinant chicken antibodies

Avian Pathology : Journal of the W.V.P.A
S I SapatsJ Ignjatovic

Abstract

Two chicken single-chain variable antibody fragments (scFv) designated scFv154 and scFv88, previously shown to react with either all or very virulent (vv) infectious bursal disease virus (IBDV) strains, respectively, were evaluated for use in an enzyme-linked immunosorbent assay (ELISA) for differentiation of vvIBDV. Specificity and sensitivity of the vvIBDV ELISA was assessed when scFv154 and scFv88 were expressed as soluble antibodies (sAb), phage antibodies (pAb) or hyper-phage antibodies (hpAb). The highest test sensitivity and specificity was obtained using hpAb154 to detect all IBDV and pAb88 to differentiate vvIBDV strains. Such an ELISA was eight to 16 times more sensitive for IBDV antigen detection than the mouse monoclonal antibody ELISA. Using field samples, the scFv ELISA was able to differentiate between flocks infected with vvIBDV and those infected with classical or variant IBDV. In one instance IBDV was detected in a flock found to be negative by the monoclonal antibody ELISA. The results showed that scFv can be utilized as highly specific and sensitive ELISA reagents for the detection and discrimination of avian pathogens.

References

Aug 17, 2000·Biosensors & Bioelectronics·P A EmanuelA Dion-Schultz
Dec 17, 2002·Current Opinion in Biotechnology·Titus Kretzschmar, Thomas von Rüden
Aug 21, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Gordana WozniakFlorian Rüker
Jul 21, 2004·Journal of Immunological Methods·Andrew R M Bradbury, James D Marks
Nov 17, 2004·Avian Pathology : Journal of the W.V.P.A·T P van den BergH Müller
Jan 15, 2005·Australian Veterinary Journal·J IgnjatovicH Westbury
Jun 1, 2000·Avian Pathology : Journal of the W.V.P.A·T P Berg

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Citations

Sep 17, 2010·Expert Review of Molecular Diagnostics·Vinayagamurthy BalamuruganRaj Kumar Singh
Nov 30, 2010·Journal of Virological Methods·Seyed A GhorashiAmir H Noormohammadi
Aug 14, 2018·Frontiers in Veterinary Science·Lorena Bustamante-CórdovaJesús Hernández

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
ELISA
ELISAs
phage display

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