An embryo lethal transgenic line manifests global expression changes and elevated protein/oil ratios in heterozygous soybean plants

PloS One
Sarah I JonesLila O Vodkin

Abstract

Understanding the molecular processes of seed development is important especially in agronomic crops that produce large amounts of nutrient reserves. Because soybean is a vital source of vegetable protein worldwide, producers are concerned about increasing the total amount of protein in the seed without substantially lowering the amount of oil, another economically important product. Here we describe a transgenic soybean line with increased protein and protein/oil ratio, containing an average of 42.2% protein vs. 38.5% in controls and with a protein/oil ratio of 2.02 vs. 1.76 in controls over several generations of greenhouse growth. Other phenotypic data show that the seeds are heavier, although there are overall lower yields per plant. We postulate these effects result from insertion site mutagenesis by the transgenic construct. As this line never achieves homozygosity and appears to be embryo lethal when homozygous, one functional copy of the gene is most likely essential for normal seed development. Global transcript analyses using RNA-Seq for 88,000 gene models over two stages of cotyledon development revealed that more genes are over-expressed in the transgenic line including ribosomal protein related genes and those in t...Continue Reading

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Datasets Mentioned

BETA
GSE130483
PRJNA540616
K01193.1

Methods Mentioned

BETA
transgenic
RNA-Seq
PCR
transgenics
dissections
Assay
electrophoresis
chip
RNA Seq

Software Mentioned

Glyma
PlasMapper
BLAST
DESeq
Auto Global
Bowtie
Phytozome
Fluidigm
Primer Express
Digital PCR Analysis

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