An endo-beta-1,4-mannanase, AkMan, from the common sea hare Aplysia kurodai

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
Umme Afsari ZahuraTakao Ojima

Abstract

A mannan-degrading enzyme was isolated from the digestive fluid of the common sea hare Aplysia kurodai by ammonium sulfate fractionation followed by conventional column chromatography. The purified enzyme, named AkMan in the present paper, showed a single band with an approximate molecular mass of 40,000 Da on SDS-PAGE and preferably degraded a linear beta-1,4-mannan from green algae Codium fragile producing tri- and disaccharides. The optimal temperature of AkMan was 55 degrees C at pH 7.0 and temperature that caused 50% inactivation of AkMan during a 20-min incubation was 52 degrees C. AkMan retained high activity at pH 4.0-7.5 and was not inactivated in such acidic pH range by the incubation at 40 degrees C for 20 min. AkMan could degrade glucomannan from konjak root and galactomannan (tara gum and guar gum) as well as the linear beta-1,4-mannan, while not carboxymethyl cellulose, agarose, dextran and xylan. These results indicate that AkMan is a typical endo-beta-1,4-mannanase (EC 3.2.1.78) splitting internal beta-1,4-mannosyl linkages of mannan. The N-terminal and internal amino-acid sequences of AkMan shared approximately 55% amino-acid identity to the corresponding sequences of an abalone beta-1,4-mannanase, HdMan, which...Continue Reading

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Citations

Aug 24, 2013·BMC Research Notes·Carolina Aguilera-GálvezRicardo Acuña-Zornosa
Jul 7, 2011·Marine Drugs·Antonio Trincone
Aug 6, 2013·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Yuya KumagaiTakao Ojima
Mar 1, 2012·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Umme Afsari ZahuraTakao Ojima
May 24, 2011·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Umme Afsari ZahuraTakao Ojima
May 24, 2011·Biochimica Et Biophysica Acta·Yuya KumagaiTadashi Hatanaka
Aug 26, 2014·Frontiers in Chemistry·Mohammad M RahmanTakao Ojima
May 28, 2014·Biotechnology and Applied Biochemistry·Ren-Kuan LiXiu-Yun Ye
Oct 3, 2012·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Kimihiko MizutaniBunzo Mikami
Feb 6, 2021·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Stuart M Linton
Aug 17, 2010·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Mohammad Matiur RahmanTakao Ojima

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