An endogenous F-box protein regulates ARGONAUTE1 in Arabidopsis thaliana.

Silence
Keith W EarleyR Scott Poethig

Abstract

ARGONAUTE1 (AGO1) mediates microRNA- and small interfering RNA-directed posttranscriptional gene silencing in Arabidopsis thaliana. Mutant alleles of SQUINT (SQN) slightly reduce AGO1 activity and have weak effects on shoot morphology. A screen for mutations that suppress the sqn phenotype produced loss-of-function mutations in the F-box gene FBW2. Mutations in FBW2 not only suppress sqn but also suppress many of the developmental phenotypes of weak, but not null, alleles of AGO1 by increasing AGO1 protein levels. Conversely, over-expression of FBW2 decreases the abundance of the AGO1 protein but not AGO1 messenger RNA, further indicating that FBW2 regulates AGO1 protein levels. fbw2 mutants have no obvious morphological phenotype, but display a reduced sensitivity to abscisic acid (ABA) that can be attributed to increased AGO1 activity. Our results indicate that FBW2 is a novel negative regulator of AGO1 and suggest that it plays a role in ABA signalling and/or response.

References

Feb 28, 1998·The EMBO Journal·K BohmertC Benning
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Nov 5, 1999·Developmental Biology·P M DonnellyN G Dengler
Oct 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·M FagardH Vaucheret
Nov 15, 2000·Trends in Plant Science·W Xiao, J Jang
Jun 19, 2001·Medicinal Research Reviews·J MyungC M Crews
Jun 11, 2003·The Plant Journal : for Cell and Molecular Biology·Eddy P RisseeuwWilliam L Crosby
Aug 2, 2003·Science·José M AlonsoJoseph R Ecker
Mar 6, 2004·Nature·Catherine A Kidner, Robert A Martienssen
Mar 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Mee Yeon ParkR Scott Poethig
Aug 6, 2005·Proceedings of the National Academy of Sciences of the United States of America·N Baumberger, D C Baulcombe
Jan 26, 2006·Proceedings of the National Academy of Sciences of the United States of America·Juliet C CoatesJim Haseloff
Jan 31, 2006·The Plant Journal : for Cell and Molecular Biology·Keith W EarleyCraig S Pikaard
Sep 29, 2006·Current Opinion in Plant Biology·Esther LechnerPascal Genschik
Jan 16, 2007·The Plant Journal : for Cell and Molecular Biology·José L Reyes, Nam-Hai Chua
Sep 18, 2007·Current Biology : CB·Diane BortolamiolVéronique Ziegler-Graff
Sep 18, 2007·Current Biology : CB·Nicolas BaumbergerDavid C Baulcombe
Dec 13, 2007·Nature Reviews. Molecular Cell Biology·Gyorgy Hutvagner, Martin J Simard
Mar 18, 2008·Cellular and Molecular Life Sciences : CMLS·M S HoH Pi
Mar 18, 2008·Plant & Cell Physiology·Atsushi TakedaYuichiro Watanabe
May 30, 2008·Trends in Plant Science·Hervé Vaucheret
Aug 12, 2008·Nature·Hank H QiYang Shi
Feb 26, 2009·Cell·Richard W Carthew, Erik J Sontheimer
Feb 26, 2009·Cell·Olivier Voinnet
Mar 18, 2009·Proceedings of the National Academy of Sciences of the United States of America·Michael R SmithR Scott Poethig
Apr 4, 2009·EMBO Reports·Allison C Mallory, Hervé Vaucheret
Feb 5, 2010·The Plant Journal : for Cell and Molecular Biology·Tibor CsorbaJózsef Burgyán

❮ Previous
Next ❯

Citations

May 21, 2013·Trends in Plant Science·Marco Incarbone, Patrice Dunoyer
Dec 21, 2011·Proceedings of the National Academy of Sciences of the United States of America·Li YangR Scott Poethig
Sep 29, 2012·Proceedings of the National Academy of Sciences of the United States of America·Benoît DerrienPascal Genschik
Aug 5, 2011·Nucleic Acids Research·Angel Emilio Martínez de AlbaHervé Vaucheret
Jul 19, 2013·Proceedings. Biological Sciences·Luke M Noble, Alex Andrianopoulos
Feb 23, 2013·International Journal of Molecular Sciences·Régis Lopes CorreaPoliane Alfenas-Zerbini
Nov 5, 2013·Biochimica Et Biophysica Acta·Angel Emilio Martínez de AlbaHervé Vaucheret
Mar 5, 2011·Annual Review of Plant Biology·Zhihua Hua, Richard D Vierstra
Mar 4, 2014·Annual Review of Plant Biology·Nicolas G Bologna, Olivier Voinnet
Nov 8, 2014·Journal of Experimental Botany·Zhiqiang XianZhengguo Li
Dec 15, 2015·Current Opinion in Virology·Tamara D Collum, James N Culver
May 28, 2013·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Gang Wu
Oct 27, 2015·Frontiers in Plant Science·Krzysztof Bobik, Tessa M Burch-Smith
Dec 23, 2014·Biochimica Et Biophysica Acta·Dileep VasudevanKunchithapadam Swaminathan
May 6, 2014·Frontiers in Plant Science·Benoît Derrien, Pascal Genschik
Sep 12, 2014·Cellular and Molecular Life Sciences : CMLS·Meng XieBin Yu
Jul 22, 2016·Molecules and Cells·Seok Keun ChoSeong Wook Yang
Oct 20, 2017·The New Phytologist·Yu YuXuemei Chen
Apr 27, 2019·The Plant Journal : for Cell and Molecular Biology·Pablo A ManavellaJavier Palatnik
Oct 20, 2019·Proceedings of the National Academy of Sciences of the United States of America·Simon MichaeliPascal Genschik
Sep 13, 2011·The Journal of Biological Chemistry·Keith W Earley, R Scott Poethig
Jun 25, 2020·Plant & Cell Physiology·Woorim YangYoo-Sun Noh
Feb 14, 2014·Plant Signaling & Behavior·Corinna Speth, Sascha Laubinger
Feb 20, 2020·Planta·Nur-Athirah Abd-HamidIsmanizan Ismail
Apr 12, 2019·Frontiers in Plant Science·Junli WangGuodong Ren
Oct 14, 2019·Molecular Plant·Delfina A RéPablo A Manavella
Jan 18, 2020·Molecular Plant·Jean Molinier
Dec 30, 2020·International Journal of Molecular Sciences·Darren Manuela, Mingli Xu
Oct 18, 2011·Plant Physiology·Yijun MengMing Chen
Aug 5, 2021·Scientific Reports·Ricardo José Gonzaga PimentaAnete Pereira de Souza

❮ Previous
Next ❯

Datasets Mentioned

BETA
AK070008
CV288647

Methods Mentioned

BETA
transgenics
transgenic
genotyping
PCR
immunoprecpitation
ubiquitination

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.