An endogenously deposited fibrin scaffold determines construct size in the surgically created arteriovenous loop chamber model of tissue engineering

Journal of Vascular Surgery
Zerina LokmicGeraldine Mitchell

Abstract

An arteriovenous loop (AVL) enclosed in a polycarbonate chamber in vivo, produces a fibrin exudate which acts as a provisional matrix for the development of a tissue engineered microcirculatory network. By administering enoxaparin sodium - an inhibitor of fibrin polymerization, the significance of fibrin scaffold formation on AVL construct size (including the AVL, fibrin scaffold, and new tissue growth into the fibrin), growth, and vascularization were assessed and compared to controls. In Sprague Dawley rats, an AVL was created on femoral vessels and inserted into a polycarbonate chamber in the groin in 3 control groups (Series I) and 3 experimental groups (Series II). Two hours before surgery and 6 hours post-surgery, saline (Series I) or enoxaparin sodium (0.6 mg/kg, Series II) was administered intra-peritoneally. Thereafter, the rats were injected daily with saline (Series I) or enoxaparin sodium (1.5 mg/kg, Series II) until construct retrieval at 3, 10, or 21 days. The retrieved constructs underwent weight and volume measurements, and morphologic/morphometric analysis of new tissue components. Enoxaparin sodium treatment resulted in the development of smaller AVL constructs at 3, 10, and 21 days. Construct weight and volum...Continue Reading

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Citations

May 14, 2014·Annual Review of Biomedical Engineering·Kareen L K CoulombeCharles E Murry
Aug 20, 2014·Tissue Engineering. Part a·Gregor BuehrerAndreas Arkudas
Jun 16, 2016·Scientific Reports·Mohit P ChhayaJan-Thorsten Schantz
Nov 13, 2019·Advanced Healthcare Materials·Jelena Rnjak-KovacinaGeraldine M Mitchell
Sep 28, 2016·Plastic and Reconstructive Surgery·Nico LeibigVolker J Schmidt
Dec 29, 2020·Frontiers in Bioengineering and Biotechnology·Thomas SpäterMatthias W Laschke

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