An endoglucanase from the anaerobic fungus Orpinomyces joyonii: characterization of the gene and its product

Canadian Journal of Microbiology
J H LiuK A Beauchemin

Abstract

An endoglucanase gene (celA) was isolated from a genomic library of the ruminal fungus Orpinomyces joyonii. DNA sequence analysis of celA revealed an intronless gene encoding a typical signal sequence, an N-terminal catalytic domain, two repeated regions linked by a short Ser/Thr-rich linker and a domain of unknown function. The deduced amino acid sequence of the catalytic domain showed homology with the family 5 cellulases. While the catalytic domain of CelA was not homologous to the catalytic domain of the endoglucanase gene (EG3) from the ruminal bacterium Fibrobacter succinogenes, the repeated regions of CelA were very similar to the noncatalytic domain of EG3. This suggests that evolutionary shuffling of endoglucanase domains might occur among bacteria and fungi within the anaerobic ecosystem of the rumen. The celA gene was expressed in Escherichia coli, and the periplasmic endoglucanase was used for the characterization studies of the enzyme. CelA exhibited both endoglucanase and xylanase activities. Its pH optimum was 4 and the temperature optimum was 40 degrees C. Deletion analysis showed that the repeated sequences and C-terminal domain of CelA were not required for enzyme activity.

References

Sep 1, 1976·Nucleic Acids Research·N Blin, D W Stafford
Feb 1, 1992·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·T M WaitsF A Greco
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Oct 1, 1990·Journal of General Microbiology·G P HazlewoodH J Gilbert
Mar 11, 1987·Nucleic Acids Research·C B Harley, R P Reynolds
Jun 11, 1986·Nucleic Acids Research·G von Heijne
Jan 1, 1995·Bio/technology·G J van Rooijen, M M Moloney

❮ Previous
Next ❯

Citations

Mar 28, 2008·Journal of Industrial Microbiology & Biotechnology·Rakhee Khandeparker, Mondher Th Numan
Jul 10, 2003·Biochimica Et Biophysica Acta·Harry R HarhangiHuub J M Op den Camp
Dec 23, 1999·Current Opinion in Genetics & Development·J Lawrence
Jul 31, 2007·Applied and Environmental Microbiology·Meng QiCecil W Forsberg
Nov 10, 2001·Annual Review of Phytopathology·U Liane Rosewich, H Corby Kistler
Jun 21, 2011·International Journal of Molecular Sciences·Xin JinLi-Ming Xia
Apr 2, 2008·Annals of the New York Academy of Sciences·Lars G Ljungdahl
Sep 7, 2007·The New Phytologist·P Frey-KlettM Tarkka
Mar 21, 2000·Molecular Biology and Evolution·S Garcia-VallvéJ Palau
Apr 16, 2020·Journal of Animal Science and Technology·Jongsoo Chang, Hyunjin Park

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.