An endoglucanase is involved in infection of rice roots by the not-cellulose-metabolizing endophyte Azoarcus sp. strain BH72

Molecular Plant-microbe Interactions : MPMI
Barbara Reinhold-HurekThomas Hurek

Abstract

The nitrogen-fixing endophyte Azoarcus sp. strain BH72 infects roots of Kallar grass and rice inter- and intra-cellularly and can spread systemically into shoots without causing symptoms of plant disease. Although cellulose or its breakdown products do not support growth, this strain expresses an endoglucanase, which might be involved in infection. Sequence analysis of eglA places the secreted 34-kDa protein into the glycosyl hydrolases family 5, with highest relatedness (40% identity) to endoglucanases of the phytopathogenic bacteria Xanthomonas campestris and Ralstonia solanacearum. Transcriptional regulation studied by eglA:: gusA fusion was not significantly affected by cellulose or its breakdown products or by microaerobiosis. Strongest induction (threefold) was obtained in bacteria grown in close vicinity to rice roots. Visible sites of expression were the emergence points of lateral roots and root tips, which are the primary regions of ingress into the root. To study the role in endophytic colonization, eglA was inactivated by transposon mutagenesis. Systemic spreading of the eglA mutant and of a pilAB mutant into the rice shoot could no longer be detected by polymerase chain reaction. Microscopic inspection of infection...Continue Reading

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Citations

Oct 24, 2006·Nature Biotechnology·Andrea KrauseAlexander Goesmann
Jan 13, 2012·Molecular Plant-microbe Interactions : MPMI·Anna BuschartBarbara Reinhold-Hurek
Aug 19, 2011·Molecular Plant-microbe Interactions : MPMI·Andrea KrauseBarbara Reinhold-Hurek
Oct 6, 2011·Molecular Plant-microbe Interactions : MPMI·A SessitschB Reinhold-Hurek
Aug 15, 2006·Molecular Plant-microbe Interactions : MPMI·Mónica Rosenblueth, Esperanza Martínez-Romero
May 18, 2007·Molecular Plant-microbe Interactions : MPMI·Melanie BöhmBarbara Reinhold-Hurek
Aug 6, 2008·Annual Review of Phytopathology·Heather L Tyler, Eric W Triplett
Jan 26, 2016·Microbiological Research·Gustavo SantoyoBernard R Glick
May 4, 2011·Current Opinion in Plant Biology·Barbara Reinhold-Hurek, Thomas Hurek
Sep 16, 2008·Trends in Microbiology·Pablo R HardoimJan Dirk van Elsas
Mar 4, 2008·Environmental Microbiology·Ernesto Ormeño-OrrilloEsperanza Martínez-Romero
Apr 1, 2010·Environmental Microbiology Reports·Claudia Sofía BurbanoThomas Hurek
Apr 30, 2015·Environmental Microbiology·Eduardo BalsanelliRose A Monteiro
Aug 6, 2015·Annual Review of Phytopathology·Barbara Reinhold-HurekThomas Hurek
Apr 16, 2015·FEMS Microbiology Ecology·Claudia Sofía BurbanoBarbara Reinhold-Hurek
Sep 3, 2008·FEMS Microbiology Ecology·Michael RothballerAnton Hartmann
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Jun 29, 2017·Molecular Plant-microbe Interactions : MPMI·Andrea KrauseBarbara Reinhold-Hurek
Mar 6, 2019·Archives of Microbiology·Pramod Kumar PandeyRaj Narain Singh Yadav
Aug 25, 2019·Archives of Microbiology·Pranjali Vishwakarma, Suresh Kumar Dubey
Aug 11, 2020·Critical Reviews in Biotechnology·Ayodeji AmobonyeSanthosh Pillai
Sep 22, 2020·Environmental Microbiology·Stéphane CompantAngela Sessitsch
Jan 10, 2018·Frontiers in Microbiology·Hongwei LiuPeer M Schenk
Sep 15, 2018·Frontiers in Microbiology·Qassim EsmaeelEssaid A Barka
Apr 24, 2019·International Journal of Molecular Sciences·Artur PinskiRobert Hasterok

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