Mar 10, 1976

An endonuclease from Escherichia coli that introduces single polynucleotide chain scissions in ultraviolet-irradiated DNA

The Journal of Biological Chemistry
M Radman

Abstract

An endonuclease that makes single polynucleotide chain scissions in ultraviolet-irradiated DNA has been purified from Escherichia coli. The activity has the following properties: (a) unirradiated DNA is attacked very little if at all; (b) single strand DNA is not attacked, whether irradiated or not; (c) there is no requirement for divalent cations and the activity is not affected by the addition of EDTA; (d) the pH optimum is approximately 7; (e) the activity is inhibited by 1 M NaCl, single strand DNA, transfer RNA and double strand DNA; (f) the sedimentation coefficient, S20,w, is approximately 2.6; (g) it is a basic protein. The enzyme is tentatively named E. coli endonuclease III. The physiological function of the endonuclease has not yet been established.

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Mentioned in this Paper

Cations, Divalent
Alkalescens-Dispar Group
Endonuclease
Triplet Codon-amino Acid Adaptor Activity
Polynucleotides
Effects of Radiation
Transfer RNA
Alkaline DNase
E. Coli Endonuclease III
Sedimentation Procedure

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