An endoplasmic reticulum protein, calreticulin, is transported into the acrosome of rat sperm

Experimental Cell Research
M NakamuraT Kobayashi

Abstract

Recently, we purified a Ca(2+)-binding protein from rat spermatogenic cells [Biochem. Biophys. Res. Commun. 176, 135-1364, 1991]. In the present study, this protein was identified as calreticulin, which is a resident protein of the endoplasmic reticulum (ER). Immunohistochemical studies revealed that calreticulin was present in the acrosome of both round spermatids and mature sperm. However, under immunoelectron microscopy, gold-particles were seen over other subcellular structures of spermatocytes, spermatids, and Sertoli cells. When the labeling density in subcellular structures of spermatids was analyzed, the acrosome was found to be most heavily labeled and the Golgi apparatus was second. The complete amino acid sequence of calreticulin, deduced from the cDNA sequence, shares a high degree of identity with that of the analogous mouse protein. The cDNA encoded a protein of 416 amino acids, including a 17-residue NH2-terminal signal sequence. The mature protein contains a KDEL sequence as an ER signal at the COOH terminus. Sperm calreticulin contained no glycosyl moiety. Northern blot analysis of RNAs from purified populations of rat spermatogenic cells indicated that the calreticulin mRNA was present in both pre- and postmei...Continue Reading

Citations

Nov 24, 1999·The Biochemical Journal·M MichalakM Opas
Aug 1, 1995·The Journal of Cell Biology·L D Walensky, S H Snyder
Feb 11, 2010·Sexual Development : Genetics, Molecular Biology, Evolution, Endocrinology, Embryology, and Pathology of Sex Determination and Differentiation·T Rhen, A Schroeder
Mar 24, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Wei GuoZhide Ding
Apr 29, 2010·Reproduction : the Official Journal of the Society for the Study of Fertility·Emma RedonPascal Sourdaine
Jun 23, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Sarah CostelloStephen Publicover
Aug 11, 1997·The Journal of Cell Biology·M WaserM Michalak
Mar 23, 1999·The Journal of Cell Biology·N MesaeliM Michalak
May 11, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Stephen C KalesBrian Dixon
Sep 1, 2007·Environmental Toxicology and Pharmacology·Guillaume PelletierIh Chu
Feb 12, 2009·Biology of Reproduction·Akihiro KawashimaNaomichi Okamura
Oct 13, 2001·Molecular Reproduction and Development·R J Muñoz-GoteraA Mújica
Dec 31, 1997·Arteriosclerosis, Thrombosis, and Vascular Biology·E DaiA Lucas
Jul 1, 1997·Journal of Cellular Biochemistry·S M JethmalaniS Y Wang
Mar 15, 2019·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Naoko KannoYukio Kato
Nov 15, 1996·The Journal of Biological Chemistry·M MichalakM Opas
Jun 15, 1994·Molecular and Cellular Biochemistry·P D NashM Michalak
Aug 15, 1996·The Journal of Experimental Zoology·S YamamotoM Nakamura
Apr 30, 1999·Physiological Reviews·A DarszonF Espinosa
Dec 14, 2004·Histochemistry and Cell Biology·Thaïs Souto-PadrónWanderley de Souza
Oct 18, 2019·Frontiers in Cell and Developmental Biology·Muhammad Babar KhawarWei Li
May 1, 2020·Molecular Reproduction and Development·Cindy RiouNadine Gérard
Nov 22, 2016·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Amin GolpourHamid Niksirat
Aug 7, 2019·The World Journal of Men's Health·Keshab Kumar KarnaJong Kwan Park
Mar 1, 1996·Molecular Immunology·T O NguyenR D Sontheimer
May 1, 1994·Trends in Cell Biology·K BurnsM Michalak
May 7, 2004·Molecular Immunology·Viviana FerreiraArturo Ferreira

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.