PMID: 9445050Jan 28, 1998Paper

An endoplasmic reticulum-targeting signal sequence enhances the immunogenicity of an immunorecessive simian virus 40 large T antigen cytotoxic T-lymphocyte epitope

Journal of Virology
T M FuS S Tevethia

Abstract

An immunological hierarchy among three H-2Db-restricted cytotoxic T lymphocyte (CTL) determinants in simian virus 40 (SV40) large T antigen (Tag) was described previously: determinants I and II/III are immunodominant, whereas determinant V is immunorecessive. To assess the immunogenicity of each determinant individually and define mechanisms that contribute to the immunorecessive nature of determinant V, we constructed a panel of recombinant vaccinia viruses (rVVs) expressing minigenes encoding these determinants in various polypeptide contexts. We found the following. (i) Immunization of mice with an rVV encoding full-length SV40 Tag resulted in priming for CTL responses to determinants I and II/III but not determinant V. (ii) rVVs encoding peptide I or II/III in the cytosol or targeted to the endoplasmic reticulum (ER) were highly antigenic and immunogenic. (iii) rVVs encoding peptide V minigenes were antigenic and immunogenic if the peptide was targeted to the ER, expressed in the cytosol with short flanking sequences, or expressed from within a self-protein, murine dihydrofolate reductase. (iv) Presentation of the nonflanked peptide V (preceded by a Met codon only) could be enhanced by using a potent inhibitor of the protea...Continue Reading

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Citations

Nov 21, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·G G ChikhM P Schutze-Redelmeier
Dec 12, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·T D Schell, S S Tevethia
Sep 24, 2004·International Journal of Cancer. Journal International Du Cancer·Pasquale GalloPaolo Monaci
Jul 9, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Pavel OtahalTodd D Schell
Mar 17, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kristie M GrebeJonathan W Yewdell
Oct 18, 2011·European Journal of Immunology·Ian GaleaStephen M Thirdborough
Sep 10, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Wolfgang PasterTamás Schweighoffer
Mar 9, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lance F BartonJohn J Monaco
Jul 5, 2005·Expert Opinion on Investigational Drugs·L L An, A Sette
Nov 6, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Helen BrosiReinhold Schirmbeck
Mar 27, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jarad J WilsonAron E Lukacher
Jan 13, 2000·Immunological Reviews·J YewdellJ R Bennink
Jun 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Christina M Ryan, Todd D Schell
Mar 20, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·C C NorburyJ W Yewdell
Mar 10, 2011·Cellular and Molecular Life Sciences : CMLS·Margarita Del ValSilvia Lázaro
Nov 24, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alan M WatsonTodd D Schell
Dec 7, 2013·Expert Review of Vaccines·Larisa I KarpenkoIgor M Belyakov
Jun 8, 1999·Annual Review of Immunology·J W Yewdell, J R Bennink
May 22, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yoshitaka YoshimuraSebastian Joyce
Jan 5, 2002·Expert Opinion on Biological Therapy·Jonathan L Bramson, Yong-Hong Wan
Sep 25, 2017·The Journal of Immunology : Official Journal of the American Association of Immunologists·Arash MemarnejadianS M Mansour Haeryfar

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