An engraved surface induces weak adherence and high proliferation of nonadherent cells and microorganisms during culture.

BioTechniques
Sunil Thomas

Abstract

When cells are cultured in a Petri dish, the adherent cells attach to the bottom of the dish; whereas, the nonadherent cells float in the culture medium. It was observed that nonadherent cells could be induced to adherent-like cells when cultured in an engraved plastic dish (biosimulator). The adherence of these cells to the engraved surface could be prevented with inhibitors specific for adhesion. It was also observed that culturing microorganisms of the environment in a biosimulator induced weak adhesion and high proliferation. Analysis of the microbiome using 16S rRNA profiling demonstrated that the biosimulator was more efficient in inducing proliferation of several phyla of microorganisms compared with culture by conventional techniques.

References

Nov 25, 1998·Proceedings of the National Academy of Sciences of the United States of America·M H PillingerG Weissmann
Jan 6, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·R GerliV Costantini
Apr 15, 2003·Journal of Cell Science·Ana I TeixeiraPaul F Nealey
Apr 16, 2004·Journal of Cardiovascular Pharmacology·Guido EiseleRainer H Böger
Aug 4, 2006·Biomaterials·Jeffrey C MohrSean P Palecek
Apr 4, 2009·Cell Motility and the Cytoskeleton·Douglas W HamiltonDonald M Brunette
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·J Gregory CaporasoRob Knight
Jul 27, 2010·Journal of Bacteriology·Floyd E DewhirstWilliam G Wade
Jun 5, 2012·Journal of Bacteriology·Eric J Stewart
Jul 12, 2012·Biopharmaceutics & Drug Disposition·Yanguang CaoWilliam J Jusko
Jan 21, 2014·Trends in Biotechnology·Jacqueline L Harding, Melissa M Reynolds
Mar 7, 2014·Nature Communications·Aniruddh SarkarJongyoon Han
May 17, 2014·Assay and Drug Development Technologies·Rasheena EdmondsonLiju Yang
Jun 12, 2014·Frontiers in Microbiology·Laam LiSebastien P Faucher
May 4, 2017·Beilstein Journal of Nanotechnology·Sara Fernández-CastillejoRosa Solà
Sep 15, 2017·Journal of Biochemistry·Susumu Tanimura, Kohsuke Takeda
Jan 19, 2018·International Journal of Molecular Sciences·Jessica Hoarau-VéchotJennifer Pasquier

❮ Previous
Next ❯

Methods Mentioned

BETA
Assay
PCR

Software Mentioned

DADA2
QIIME
Bcl2Fastq
AnCom

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.