An enhanced isothermal amplification assay for viral detection

Nature Communications
Jason QianMichael Springer

Abstract

Rapid, inexpensive, robust diagnostics are essential to control the spread of infectious diseases. Current state of the art diagnostics are highly sensitive and specific, but slow, and require expensive equipment. Here we report the development of a molecular diagnostic test for SARS-CoV-2 based on an enhanced recombinase polymerase amplification (eRPA) reaction. eRPA has a detection limit on patient samples down to 5 viral copies, requires minimal instrumentation, and is highly scalable and inexpensive. eRPA does not cross-react with other common coronaviruses, does not require RNA purification, and takes ~45 min from sample collection to results. eRPA represents a first step toward at-home SARS-CoV-2 detection and can be adapted to future viruses within days of genomic sequence availability.

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Citations

Nov 22, 2020·Nature Communications·Jon Arizti-SanzCameron Myhrvold
Mar 9, 2021·ACS Sensors·Yongzhong JiangDai-Wen Pang
Feb 27, 2021·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·John J SchellenbergYoav Keynan
Mar 20, 2021·Analytica Chimica Acta·Junmin LiShan Li
Apr 6, 2021·Journal of Pharmaceutical Analysis·Congshan JiangShemin Lu
May 14, 2021·Journal of Virological Methods·Mary E PettitMichael Springer
Jun 25, 2021·BioTechniques·Deguo WangYanhong Liu
Jun 29, 2021·Analytical Chemistry·Christopher P MancusoMichael Springer
Jul 3, 2021·International Journal of Molecular Sciences·Hee Min YooSeil Kim
Aug 27, 2021·Signal Transduction and Targeted Therapy·Zirui MengBinwu Ying
Oct 9, 2021·Advanced Materials·Zhaowei ZhangUtkan Demirci
Nov 11, 2021·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Prakash GhoshDinesh Mondal

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Methods Mentioned

BETA
PCR
in vitro transcription

Software Mentioned

Clustal Omega
VTM
BLAST
IGV
Samtools
eRPA
BLAST +
RNaseAlert
AccuPlex
R

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