An enhanced Lactobacillus reuteri biofilm formulation that increases protection against experimental necrotizing enterocolitis

American Journal of Physiology. Gastrointestinal and Liver Physiology
Jacob K OlsonGail E Besner

Abstract

One significant drawback of current probiotic therapy for the prevention of necrotizing enterocolitis (NEC) is the need for at least daily administration because of poor probiotic persistence after enteral administration, increasing the risk of the probiotic bacteria causing bacteremia or sepsis if the intestines are already compromised. We previously showed that the effectiveness of Lactobacillus reuteri ( Lr) in preventing NEC is enhanced when Lr is grown as a biofilm on the surface of dextranomer microspheres (DM). Here we sought to test the efficacy of Lr administration by manipulating the Lr biofilm state with the addition of biofilm-promoting substances (sucrose and maltose) to DM or by mutating the Lr gtfW gene (encoding an enzyme central to biofilm production). Using an animal model of NEC, we determined that Lr adhered to sucrose- or maltose-loaded DM significantly reduced histologic injury, improved host survival, decreased intestinal permeability, reduced intestinal inflammation, and altered the gut microbiome compared with Lr adhered to unloaded DM. These effects were abolished when DM or GtfW were absent from the Lr inoculum. This demonstrates that a single dose of Lr in its biofilm state decreases NEC incidence. I...Continue Reading

References

Jan 1, 1976·Scandinavian Journal of Plastic and Reconstructive Surgery·S JacobssonU Rothman
May 1, 1989·Antimicrobial Agents and Chemotherapy·T L Talarico, W J Dobrogosz
Oct 1, 1974·Journal of Pediatric Surgery·B BarlowJ N Schullinger
Nov 27, 1999·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·A B Hoyos
Nov 1, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Minna K SalminenAsko Järvinen
Oct 15, 2003·Clinical Microbiology Reviews·Gregor ReidJohn K McCormick
Feb 10, 2004·Applied and Environmental Microbiology·Nana ValeurKarin Ladefoged
Apr 16, 2004·Journal of Pediatric Gastroenterology and Nutrition·Anjali N KunzMary P Fairchok
Jan 5, 2005·Pediatrics·Michael H LandAvinash K Shetty
Mar 8, 2005·The Pediatric Infectious Disease Journal·Mary Ann De GrooteNorman R Pace
Mar 14, 2007·The Journal of Allergy and Clinical Immunology·Thomas R AbrahamssonGöran Oldaeus
Nov 24, 2007·Journal of Food Science·W K Ding, N P Shah
Aug 9, 2008·Surgical Infections·Catherine J HunterNemani V Prasadarao
Sep 27, 2008·Annual Review of Medicine·Marion C W Henry, R Lawrence Moss
Jan 17, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·O SchreiberL Holm
Feb 13, 2009·BMC Microbiology·Sara E Jones, James Versalovic
Aug 28, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Yuying LiuJon Marc Rhoads
Jul 15, 2011·International Journal of Antimicrobial Agents·Stephen E MshanaCan Imirzalioglu
Nov 8, 2011·Archives of Disease in Childhood. Fetal and Neonatal Edition·Andreas JenkeStefan Wirth
Dec 31, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Yuying LiuJon Marc Rhoads
Jun 13, 2012·Journal of Clinical Gastroenterology·Francisco GuarnerUNKNOWN World Gastroenterology Organization
Sep 27, 2012·Archives of Disease in Childhood. Fetal and Neonatal Edition·Janet Elizabeth BerringtonStephen Paul Cummings
Oct 30, 2012·Gastroenterology Clinics of North America·Federica MolinaroGiovanni Musso
Mar 29, 2013·Journal of Pediatric Gastroenterology and Nutrition·Shu-Fen WuMichael Caplan
Apr 25, 2013·Seminars in Pediatric Surgery·Anatoly GrishinHenri R Ford
Dec 20, 2013·Journal of Developmental Origins of Health and Disease·M Weng, W A Walker
Mar 15, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Meiqian WengW Allan Walker
Mar 19, 2014·Pediatrics·Pedro Gutierrez-CastrellonMinerva Parra
Apr 12, 2014·The Cochrane Database of Systematic Reviews·Khalid AlFaleh, Jasim Anabrees
Apr 20, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Misty GoodDavid J Hackam

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Citations

Jan 22, 2019·Current Opinion in Pediatrics·Mark A Underwood
Apr 25, 2019·Minerva pediatrica·Mark A Underwood
Oct 21, 2018·Nutrients·Yuying LiuJ Marc Rhoads
Mar 15, 2020·Nutrients·Jeffrey D Galley, Gail E Besner
Aug 29, 2020·Pediatric Research·Troy A MarkelMisty Good
Aug 29, 2020·Pediatric Research·Rita D ShelbySteven J McElroy
Aug 9, 2020·Disease Models & Mechanisms·Mark L KovlerDavid J Hackam
Apr 17, 2019·Digestive Diseases and Sciences·Kewei WangKarl G Sylvester
Sep 15, 2019·Applied and Environmental Microbiology·Monchaya RattanaprasertRobert W Hutkins
Jan 27, 2021·Acta Veterinaria Hungarica·Bohumil BielikDagmar Mudroňová

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Methods Mentioned

BETA
scanning electron microscopy
cesarean section
electrophoresis
PCR

Software Mentioned

TORNADO
SAS
QIIME
IM
Fluidigm Access
BLAST
R
GraphPad Prism

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