An enhanced microsatellite map of diploid Fragaria

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
D J SargentN H Battey

Abstract

A total of 45 microsatellites (SSRs) were developed for mapping in Fragaria. They included 31 newly isolated codominant genomic SSRs from F. nubicola and a further 14 SSRs, derived from an expressed sequence tagged library (EST-SSRs) of the cultivated strawberry, F. x ananassa. These, and an additional 64 previously characterised but unmapped SSRs and EST-SSRs, were scored in the diploid Fragaria interspecific F2 mapping population (FVxFN) derived from a cross between F. vesca 815 and F. nubicola 601. The cosegregation data of these 109 SSRs, and of 73 previously mapped molecular markers, were used to elaborate an enhanced linkage map. The map is composed of 182 molecular markers (175 microsatellites, six gene specific markers and one sequence-characterised amplified region) and spans 424 cM over seven linkage groups. The average marker spacing is 2.3 cM/marker and the map now contains just eight gaps longer than 10 cM. The transferability of the new SSR markers to the cultivated strawberry was demonstrated using eight cultivars. Because of the transferable nature of these markers, the map produced will provide a useful reference framework for the development of linkage maps of the cultivated strawberry and for the development ...Continue Reading

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Citations

Nov 9, 2006·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·D J SargentK R Tobutt
Sep 30, 2009·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·E PortisS Lanteri
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Jan 1, 2014·Horticulture Research·Sara LonghiDaniel J Sargent

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