An enhanced-sensitivity branched-DNA assay for quantification of human immunodeficiency virus type 1 RNA in plasma.

Journal of Clinical Microbiology
D KernJ Todd

Abstract

The quantification of human immunodeficiency virus type 1 (HIV-1) RNA has facilitated clinical research and expedited the development of antiretroviral drugs. The branched-DNA (bDNA) assay provides a reliable method for the quantification of HIV-1 RNA in human plasma and is considered one of the most reproducible assays ready for use in clinical trials. A series of oligonucleotide probe design and solution changes have been developed to enhance the sensitivity of the bDNA assay while maintaining its performance characteristics. Among the changes incorporated into the enhanced-sensitivity bDNA (ES bDNA) assay to reduce the background level and enhance the signal are the use of shorter overhang sequences of target probes for capture, the cruciform design of target probes for amplification, and the addition of preamplifier molecules. The ES bDNA assay is at least 20-fold more sensitive than the first-generation bDNA assay, yet it maintains a high level of accuracy, linearity, and reproducibility. Further, quantification values obtained with the ES bDNA assay and the first-generation bDNA assay are highly correlated, thus allowing for meaningful comparisons of HIV-1 RNA levels in specimens tested with either assay. The ES bDNA assa...Continue Reading

References

Jul 1, 1992·AIDS Research and Human Retroviruses·S Aoki-SeiH Mitsuya
Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M R Webb
Sep 1, 1966·Journal of Molecular Biology·M M Warshaw, I Tinoco
Dec 7, 1995·The New England Journal of Medicine·M MarkowitzJ M Leonard
Apr 15, 1995·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·C PachlB Irvine
Apr 15, 1995·Annals of Internal Medicine·J W MellorsP Gupta

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Citations

Apr 20, 2000·AIDS Research and Human Retroviruses·A HolguínV Soriano
Aug 10, 2000·Journal of Acquired Immune Deficiency Syndromes : JAIDS·A Machuca, V Soriano
Sep 15, 2005·HIV Medicine·E LospitaoA Holguín
Dec 3, 2014·Nature Reviews. Genetics·Nicola CrosettoAlexander van Oudenaarden
Aug 21, 2019·Nature Biotechnology·Sinem K SakaPeng Yin
Apr 17, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A N PlayerJ A Kolberg
Jan 25, 2007·Clinical Chemistry and Laboratory Medicine : CCLM·Patrick BraunHeribert Knechten
Jan 15, 2014·Mycoses·Bosco Christiano Maciel da SilvaWalderez Gambale
Oct 6, 2000·AIDS Research and Human Retroviruses·A HolguínV Soriano
Aug 13, 2003·Biological & Pharmaceutical Bulletin·Akiko IwataTeruhide Yamaguchi
Aug 2, 2006·American Journal of Clinical Pathology·Gregory J Tsongalis
Nov 16, 2011·Angewandte Chemie·Anna BaccaroAndreas Marx
May 24, 2019·Scientific Reports·Chenglong XiaXiaowei Zhuang
Jul 6, 2000·Journal of Acquired Immune Deficiency Syndromes : JAIDS·C BrionesJ González-Lahoz
Aug 20, 2008·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Joon-Yong ChungStephen M Hewitt
May 19, 2011·Experimental Dermatology·Yonghu SunFuren Zhang
Nov 26, 2015·Nucleic Acid Therapeutics·Andrew H ColesAnastasia Khvorova
Dec 3, 2016·Proceedings of the National Academy of Sciences of the United States of America·Jeffrey R MoffittXiaowei Zhuang
May 23, 2002·AIDS Research and Human Retroviruses·Africa HolguínVincent Soriano
Sep 12, 2006·Nature Biotechnology·Roger D CanalesFederico M Goodsaid
Aug 21, 2004·Clinical Chemistry·Scott C JohnsonJames R Prudent
Aug 11, 1999·Molecular and Cellular Probes·S S IqbalJ J Valdes
Nov 13, 2018·Nature Biotechnology·Sara H RouhanifardArjun Raj
Oct 22, 1998·Mathematical Biosciences·J E MittlerA S Perelson

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