PMID: 2503812Jul 11, 1989Paper

An enhancer associated with the mouse immunoglobulin lambda 1 gene is specific for lambda light chain producing cells

Nucleic Acids Research
L T Bich-Thuy, C Queen

Abstract

We have developed a system to study transcriptional regulation of the lambda immunoglobulin gene in a natural setting -- lambda light chain producing lymphoid cells. This assay system has allowed the detection of an enhancer element located 3' of the lambda gene coding sequence. The enhancer can stimulate transcription from the lambda promoter as well as from other immunoglobulin and unrelated promoters. Like all enhancers, the lambda enhancer can function in either orientation with respect to a promoter, but it is significantly more active in one orientation than in the other. The lambda enhancer is unusual in spanning at least 4000 bp of DNA sequence and containing several distinct subelements that have independent enhancer activity. The enhancer is also remarkable because it functions in lambda light chain producing cells but not in kappa chain producing cells. This fact can be interpreted to support a model of immunoglobulin gene rearrangement in which rearrangement follows and depends on transcriptional activation.

References

Jul 24, 1986·Nature·J V GarciaC Queen
Jul 24, 1987·Nucleic Acids Research·G KolliasF Grosveld
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·J E HesseG Felsenfeld
Apr 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·J Shine, L Dalgarno
Nov 1, 1984·Hoppe-Seyler's Zeitschrift für physiologische Chemie·F G FalknerH G Zachau
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·D Picard, W Schaffner
Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·V T OiP Berg
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·B BlombergS Tonegawa

❮ Previous
Next ❯

Related Concepts

Related Feeds

B cells: Gene Expression

B lymphocytes are white blood cells that play a role in the adaptive immune system by secreting antibodies. Here is the latest research on gene expression in B cells.

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.