An enhancer element at the Igf2/H19 locus drives gene expression in both imprinted and non-imprinted tissues

Developmental Biology
M CharalambousAndrew Ward

Abstract

The insulin-like growth factor 2 (Igf2) gene encodes a potent growth factor that is expressed in multiple tissues during embryonic development. Expression at this locus is mediated by genomic imprinting. In the developing endodermal tissues, imprinting of Igf2 is mediated by the interaction of a set of enhancers downstream of the linked H19 gene with a differentially methylated domain (DMD) that lies approximately 2-4 kb upstream of H19 that has a boundary or insulator function in the hypomethylated state. In the remainder of tissues that express Igf2 and H19, the cis elements that drive their correct expression and imprinting are not well understood. In addition, enhancers driving expression of Igf2 in the choroid plexus and leptomeninges, tissues where the gene is thought not to be imprinted, have not been isolated. Here we show that biallelic (non-imprinted) expression within the choroid plexus is restricted to the epithelium, and we provide evidence that a conserved intergenic region functions as an enhancer for Igf2 both in tissues where the gene is imprinted, and where Igf2 is biallelically expressed. The presence of an enhancer for imprinted tissues in the intergenic region argues for the existence of imprinting controls...Continue Reading

References

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Jun 28, 2003·Proceedings of the National Academy of Sciences of the United States of America·Marika CharalambousAndrew Ward

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Citations

Jul 4, 2006·Proceedings of the National Academy of Sciences of the United States of America·Sreenivasulu KurukutiRolf Ohlsson
Apr 11, 2009·Comparative and Functional Genomics·Elias Daura-OllerAntoni Romeu
Jun 29, 2005·The Journal of Experimental Medicine·Jens DerbinskiBruno Kyewski
Sep 17, 2008·Molecular and Cellular Biology·Nathalie BerteauxEric Adriaenssens
Apr 16, 2013·Nucleic Acids Research·Yoko ItoAdele Murrell
Jun 14, 2008·Yi chuan = Hereditas·Rong-Rong YangXiang-Yun Li
Aug 8, 2013·Nature Communications·Mikael Allan MikaelssonTrevor Humby
Mar 14, 2009·Animal Reproduction Science·Carol Lynn CurchoeX Cindy Tian
Apr 18, 2014·Cell Proliferation·M NordinA Ward
May 21, 2010·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Anne GaboryLuisa Dandolo
Nov 1, 2005·Journal of Theoretical Biology·Jon F Wilkins
Apr 14, 2012·Biochimica Et Biophysica Acta·Marika Charalambous, Arturo Hernandez
Jan 1, 2011·Clinical Epigenetics·L Serman, D Dodig
Mar 17, 2015·PloS One·Bambarendage P U PereraJoomyeong Kim
Dec 7, 2018·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jon N BuzzelliTrevelyan R Menheniott
Apr 1, 2006·Cytogenetic and Genome Research·A GaboryL Dandolo
Oct 18, 2007·Current Opinion in Endocrinology, Diabetes, and Obesity·Marika CharalambousAnne C Ferguson-Smith

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