Abstract
Protozoan parasites of the genus Entamoeba possess a considerable number of cysteine peptidases (CPs), the function of most of these molecules for amoeba biology needs to be established. In order to determine whether CPs may play a role during Entamoeba stage conversion from trophozoites into cysts and vice versa, expression of cp genes was analysed in the reptilian parasite Entamoeba invadens, a model organism for studying Entamoeba cyst development. By homology search, 28 papain-like cp genes were identified in public E. invadens genome databases. For eight of these genes the expression profiles during stage conversion was determined. By Northern blot analysis, transcripts for eicp-a9, -b7, -b8 and -c2, respectively, were detected neither in trophozoites or cysts nor at any of the point of times analysed during stage conversion. On the other hand, eicp-a5 is constitutively expressed during all developmental stages, whereas eicp-a3 and eicp-a11, respectively, are trophozoite-specific. Only eicp-b9 was found to be cyst-specific as it is expressed exclusively 18 to 28 h after cyst induction. Cyst-specific expression was confirmed by immunofluorescence microscopy of the corresponding protein EiCP-B9. In immature cysts, the molecu...Continue Reading
References
Jan 1, 1978·Transactions of the Royal Society of Tropical Medicine and Hygiene·L S DiamondC C Cunnick
Sep 1, 1994·Molecular and Biochemical Parasitology·L SanchezD Eichinger
Apr 1, 1997·Molecular and Biochemical Parasitology·H de la VegaJ Samuelson
May 2, 1997·Cell·W WardJ H McKerrow
Sep 22, 1999·European Journal of Biochemistry·J GonzalezD Eichinger
Apr 13, 2000·Biochimica Et Biophysica Acta·A SchlüterJ Clos
Jan 5, 2002·The Journal of Biological Chemistry·María C TouzHugo D Luján
Feb 19, 2002·Molecular and Biochemical Parasitology·M Sajid, J H McKerrow
Jun 5, 2002·Annual Review of Biochemistry·Michael Klemba, Daniel E Goldberg
Dec 6, 2002·The Journal of Biological Chemistry·Maria C TouzTheodore E Nash
Jun 12, 2003·Molecular and Biochemical Parasitology·Zheng WangBrendan Loftus
Dec 11, 2003·Eukaryotic Cell·Eduardo VillaloboLoïc Morin
Nov 24, 2004·Protist·Eduardo Campos-GóngoraEgbert Tannich
Jan 11, 2005·Experimental Parasitology·Asao MakiokaTsutomu Takeuchi
Feb 25, 2005·Nature·Brendan LoftusNeil Hall
May 10, 2006·Eukaryotic Cell·Katrina L Van DellenJohn Samuelson
Jun 29, 2006·Molecular Microbiology·Roderick A WilliamsGraham H Coombs
Jun 15, 2007·BMC Genomics·Manuela TillackIris Bruchhaus
Oct 10, 2007·Infection and Immunity·Karina PicazarriTomoyoshi Nozaki
Dec 8, 2007·Advances in Parasitology·C G ClarkN Hall
Citations
Feb 12, 2013·Eukaryotic Cell·Jung-Yub LeeYeonchul Hong
Mar 1, 2012·Eukaryotic Cell·Emilie FouqueYann Héchard
Aug 29, 2013·Parasites & Vectors·Ramón Ocádiz-RuizMario A Rodríguez
May 17, 2012·PLoS Neglected Tropical Diseases·Ibne Karim M AliWilliam A Petri
Sep 17, 2013·PloS One·Aleyla Escueta De CádizTomoyoshi Nozaki
Jul 7, 2009·PLoS Pathogens·Anirban ChatterjeeJohn Samuelson
Mar 12, 2013·Journal of Tropical Medicine·Jesús Serrano-LunaMireya de la Garza
Feb 17, 2009·Trends in Parasitology·Carol A Gilchrist, William A Petri
Jun 20, 2012·The Journal of Eukaryotic Microbiology·Yoichiro SogameTatsuomi Matsuoka
Oct 19, 2013·The Journal of Eukaryotic Microbiology·Yoichiro SogameTatsuomi Matsuoka
Mar 25, 2015·Parasitology·Sonja Frölich, Michael Wallach
Mar 29, 2011·Protist·Mirela ŠarićHenning Scholze
Aug 25, 2012·Molecular and Biochemical Parasitology·Eun-Kyung MoonHyun-Hee Kong
Mar 31, 2012·FEMS Microbiology Letters·Yoichiro SogameTatsuomi Matsuoka
Jun 16, 2015·PloS One·Yu-Ran LeeYeonchul Hong
Aug 24, 2018·PLoS Neglected Tropical Diseases·Jair L Siqueira-NetoPhilip J Rosenthal
Nov 14, 2018·The Korean Journal of Parasitology·Yeonchul HongByoung-Kuk Na
Mar 16, 2021·Frontiers in Cellular and Infection Microbiology·Manish T Shrivastav Somlata
Apr 4, 2021·Pathogens·Hương Giang LêByoung-Kuk Na