An Entamoeba cysteine peptidase specifically expressed during encystation

Parasitology International
Frank EbertI Bruchhaus

Abstract

Protozoan parasites of the genus Entamoeba possess a considerable number of cysteine peptidases (CPs), the function of most of these molecules for amoeba biology needs to be established. In order to determine whether CPs may play a role during Entamoeba stage conversion from trophozoites into cysts and vice versa, expression of cp genes was analysed in the reptilian parasite Entamoeba invadens, a model organism for studying Entamoeba cyst development. By homology search, 28 papain-like cp genes were identified in public E. invadens genome databases. For eight of these genes the expression profiles during stage conversion was determined. By Northern blot analysis, transcripts for eicp-a9, -b7, -b8 and -c2, respectively, were detected neither in trophozoites or cysts nor at any of the point of times analysed during stage conversion. On the other hand, eicp-a5 is constitutively expressed during all developmental stages, whereas eicp-a3 and eicp-a11, respectively, are trophozoite-specific. Only eicp-b9 was found to be cyst-specific as it is expressed exclusively 18 to 28 h after cyst induction. Cyst-specific expression was confirmed by immunofluorescence microscopy of the corresponding protein EiCP-B9. In immature cysts, the molecu...Continue Reading

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Citations

Aug 29, 2013·Parasites & Vectors·Ramón Ocádiz-RuizMario A Rodríguez
May 17, 2012·PLoS Neglected Tropical Diseases·Ibne Karim M AliWilliam A Petri
Sep 17, 2013·PloS One·Aleyla Escueta De CádizTomoyoshi Nozaki
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Feb 17, 2009·Trends in Parasitology·Carol A Gilchrist, William A Petri
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Mar 25, 2015·Parasitology·Sonja Frölich, Michael Wallach
Aug 25, 2012·Molecular and Biochemical Parasitology·Eun-Kyung MoonHyun-Hee Kong
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Nov 14, 2018·The Korean Journal of Parasitology·Yeonchul HongByoung-Kuk Na
Mar 16, 2021·Frontiers in Cellular and Infection Microbiology·Manish T Shrivastav Somlata

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